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Author(s): Guillaume L. Erny
Marzieh Moeenfard
Arminda Alves
Title: Liquid chromatography with diode array detection combined with spectral deconvolution for the analysis of some diterpene esters in Arabica coffee brew
Issue Date: 2015
Abstract: In this manuscript, the separation of kahweol and cafestol esters from Arabica coffee brewswas investigated using liquid chromatography with a diode array detector. When detectedin conjunction, cafestol, and kahweol esters were eluted together, but, after optimization,the kahweol esters could be selectively detected by setting the wavelength at 290 nm toallow their quantification. Such an approach was not possible for the cafestol esters, andspectral deconvolution was used to obtain deconvoluted chromatograms. In each of thosechromatograms, the four esters were baseline separated allowing for the quantification ofthe eight targeted compounds. Because kahweol esters could be quantified either usingthe chromatogram obtained by setting the wavelength at 290 nm or using the deconvoluted chromatogram, those compounds were used to compare the analytical performances.Slightly better limits of detection were obtained using the deconvoluted chromatogram.Identical concentrations were found in a real sample with both approaches. The peak areasin the deconvoluted chromatograms were repeatable (intraday repeatability of 0.8%, interday repeatability of 1.0%). This work demonstrates the accuracy of spectral deconvolutionwhen using liquid chromatography to mathematically separate coeluting compounds usingthe full spectra recorded by a diode array detector.
Subject: Ciências Tecnológicas, Ciências da engenharia e tecnologias
Technological sciences, Engineering and technology
Scientific areas: Ciências da engenharia e tecnologias
Engineering and technology
Related Information: info:eu-repo/grantAgreement/Autoriadade de Gestão do Programa Operacional Regional do Norte/Programas Integrados de IC&DT/NORTE-07-0124-FEDER-000025/(Bio) Chemical Engineering: Multi-Scale Approaches for Sustainable Environment and Health/LEPAE/CEFT - RL2
info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projetos Estratégicos/PEst-C/EQB/UI0511/2013/PROJECTO ESTRATÉGICO - UI 511 - 2013-2014/UI0511
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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