Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/103594
Author(s): Túlio I. S. Oliveira
Marcela Oliveira
Subramanian Viswanathan
M. Fátima Barroso
Luísa Barreiros
Olga C. Nunes
José A. Rodrigues
Pedro de Lima Neto
Selma E. Mazzetto
Simone Morais
Cristina Delerue Matos
Title: Molinate quantification in environmental water by a glutathione-S-transferase based biosensor
Issue Date: 2013
Abstract: A glutathione-S-transferase (GST) based biosensor was developed to quantify the thiocarbamate herbicide molinate in environmental water. The biosensor construction was based on GST immobilization onto a glassy carbon electrode via aminosilane-glutaraldehyde covalent attachment. The principle supporting the use of this biosensor consists of the GST inhibition process promoted by molinate. Differential pulse voltammetry was used to obtain a calibration curve for molinate concentration, ranging from 0.19 to 7.9 mg L-1 and presenting a detection limit of 0.064 mg L-1. The developed biosensor is stable, and reusable during 15 days. The GST-based biosensor was successfully applied to quantify molinate in rice paddy field floodwater samples. The results achieved with the developed biosensor were in accordance with those obtained by high performance liquid chromatography. The proposed device is suitable for screening environmental water analysis and, since no sample preparation is required, it can be used in situ and in real-time measurements.
Subject: Química
Chemical sciences
Scientific areas: Ciências exactas e naturais::Química
Natural sciences::Chemical sciences
URI: https://hdl.handle.net/10216/103594
Related Information: info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projetos Estratégicos/PEst-C/EQB/UI0511/2013/PROJECTO ESTRATÉGICO - UI 511 - 2013-2014/UI0511
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FCUP - Artigo em Revista Científica Internacional
FEUP - Artigo em Revista Científica Internacional

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