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dc.creatorNuno F. F. Moreira
dc.creatorJosé M. Sousa
dc.creatorGonçalo Macedo
dc.creatorAna R. Ribeiro
dc.creatorLuisa Barreiros
dc.creatorMarta Pedrosa
dc.creatorJoaquim L. Faria
dc.creatorM. Fernando R. Pereira
dc.creatorSérgio Castro Silva
dc.creatorMarcela A. Segundo
dc.creatorCélia M. Manaia
dc.creatorOlga C. Nunes
dc.creatorAdrián M. T. Silva
dc.description.abstractPhotocatalytic ozonation was employed for the first time in continuous mode with TiO2-coated glass Raschig rings and light emitting diodes (LEDs) to treat urban wastewater as well as surface water collected from the supply area of a drinking water treatment plant (DWTP). Different levels of contamination and types of contaminants were considered in this work, including chemical priority substances (PSs) and contaminants of emerging concern (CECs), as well as potential human opportunistic antibiotic resistant bacteria and their genes (ARB&ARG). Photocatalytic ozonation was more effective than single ozonation (or even than TiO2 catalytic ozonation) in the degradation of typical reaction by-products (such as oxalic acid), and more effective than photocatalysis to remove the parent micro pollutants determined in urban wastewater. In fact, only fluoxetine, clarithromycin, erythromycin and 17-alpha-ethinylestradiol (EE2) were detected after photocatalytic ozonation, by using solid-phase extraction (SPE) pre-concentration and LC-MS/MS analysis. In surface water, this treatment allowed the removal of all determined micropollutants to levels below the limit of detection (0.01-0.20 ng L-1). The efficiency of this process was then assessed based on the capacity to remove different groups of cultivable microorganisms and housekeeping (16S rRNA) and antibiotic resistance or related genes (intl1, blaTEM, qnrS, sul1). Photocatalytic ozonation was observed to efficiently remove microorganisms and ARGs. Although after storage total heterotrophic and ARB (to ciprofloxacin, gentamicin, meropenem), fungi, and the genes 16S rRNA and intl1, increased to values close to the pre-treatment levels, the ARGs (blaTEM, qnrS and sul1) were reduced to levels below/close to the quantification limit even after 3-days storage of treated surface water or wastewater. Yeast estrogen screen (YES), thiazolyl blue tetrazolium reduction (MTT) and lactate dehydrogenase (LDH) assays were also performed before and after photo catalytic ozonation to evaluate the potential estrogenic activity, the cellular metabolic activity and the cell viability. Compounds with estrogenic effects and significant differences concerning cell viability were not observed in any case. A slight cytotoxicity was only detected for Caco-2 and hCMEC/D3 cell lines after treatment of the urban wastewater, but not for L929 fibroblasts.
dc.relationinfo:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projetos Estratégicos/UID/EQU/50020/2013- POCI-01-0145-FEDER-006984/Laboratório de Processos de Separação e Reação - Laboratório de Catálise e Materiais/LSRE-LCM
dc.relationinfo:eu-repo/grantAgreement/Agência de Inovação, S.A./Projectos de I&DT em Co-Promoção/ SI IDT - 38900/2012 F3 /Novo equipamento envolvendo processos catalíticos integrados para tratamento de poluentes orgânicos e desinfeção de águas/NEPCAT
dc.relationinfo:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projetos Estratégicos/UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939/Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia/LEPABE
dc.relationinfo:eu-repo/grantAgreement/Autoriadade de Gestão do Programa Operacional Regional do Norte/Programas Integrados de IC&DT/NORTE-07-0124-FEDER-000025/(Bio) Chemical Engineering: Multi-Scale Approaches for Sustainable Environment and Health/LEPAE/CEFT - RL2
dc.relationinfo:eu-repo/grantAgreement/COMISSÃO EUROPEIA/COST - European Cooperation in Science and Technology/ES1403PT//NEREUS
dc.titlePhotocatalytic ozonation of urban wastewater and surface water using immobilized TiO2 with LEDs: Micropollutants, antibiotic resistance genes and estrogenic activity
dc.typeArtigo em Revista Científica Internacional
dc.contributor.uportoFaculdade de Farmácia
dc.contributor.uportoFaculdade de Engenharia
Appears in Collections:FEUP - Artigo em Revista Científica Internacional
FFUP - Artigo em Revista Científica Internacional

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