Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/103578
Author(s): | Joana Barros Liliana Grenho Cândida M. Manuel Carla Ferreira Luís F. Melo Olga C. Nunes Fernando J. Monteiro Maria P. Ferraz |
Title: | A modular reactor to simulate biofilm development in orthopedic materials |
Issue Date: | 2013 |
Abstract: | Surfaces of medical implants are generally designed to encourage soft- and/or hard-tissue adherence, eventually leading to tissue- or osseo-integration. Unfortunately, this feature may also encourage bacterial adhesion and biofilm formation. To understand the mechanisms of bone tissue infection associated with contaminated biomaterials, a detailed understanding of bacterial adhesion and subsequent biofilm formation on biomaterial surfaces is needed. In this study, a continuous-flow modular reactor composed of several modular units placed in parallel was designed to evaluate the activity of circulating bacterial suspensions and thus their predilection for biofilm formation during 72 h of incubation. Hydroxyapatite discs were placed in each modular unit and then removed at fixed times to quantify biofilm accumulation. Biofilm formation on each replicate of material, unchanged in structure, morphology, or cell density, was reproducibly observed. The modular reactor therefore proved to be a useful tool for following mature biofilm formation on different surfaces and under conditions similar to those prevailing near human-bone implants. |
Subject: | Ciências Naturais, Ciências biológicas Natural sciences, Biological sciences |
Scientific areas: | Ciências exactas e naturais::Ciências biológicas Natural sciences::Biological sciences |
URI: | https://hdl.handle.net/10216/103578 |
Related Information: | info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e a Tecnologia/Projectos de I&DT em Todos os Domínios Científicos/PTDC/SAU-BMA/111233/2009/NaNOBiofilm - Superfícies de nanohidroxiapatite com caracteristicas antiobacterianas para prevenção de infecção óssea associada a biofilmes/NaNOBiofilm |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | restrictedAccess |
Appears in Collections: | FEUP - Artigo em Revista Científica Internacional |
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File | Description | Size | Format | |
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93607.pdf Restricted Access | Artigo original publicado | 390.8 kB | Adobe PDF | Request a copy from the Author(s) |
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