Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/102630
Author(s): Filipa Castro
António Ferreira
José A. Teixeira
Fernando Rocha
Title: Protein Crystallization As a Process Step in a Novel Meso Oscillatory Flow Reactor: Study of Lysozyme Phase Behavior
Issue Date: 2016
Abstract: In the present work, it is reported for the first time the study of the applicability of a novel meso oscillatory flow reactor (meso-OFR) for protein crystallization as a process step. Crystallization assays carried out in the designed device enabled to derive a two-dimensional lysozyme phase diagram (lysozyme concentration against sodium chloride concentration). Results evidence the formation of several types of crystals (different size and shape), with a strong influence of salt concentration on crystal shape. Results also show that lysozyme remains active at the end of the experiments. Furthermore, it was possible to verify the reduction of the metastability zone when compared to lysozyme crystallization conducted under quiescent conditions. Induction times were also measured by online monitoring of the turbidity of the crystallization solution, obtained values being between 41 and 900 min. Beyond providing improved understanding of protein phase behavior under oscillatory flow mixing, the results are very promising regarding the feasibility of the designed methodology for protein crystallization as a process step.
Subject: Engenharia química, Engenharia química
Chemical engineering, Chemical engineering
Scientific areas: Ciências da engenharia e tecnologias::Engenharia química
Engineering and technology::Chemical engineering
URI: https://hdl.handle.net/10216/102630
Related Information: info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Investigador FCT/IF/01087/2014/CP1249/CT0001/Projeto de investigação exploratória/IF/01087/2014/CP1249/CT0001
info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projetos Estratégicos/UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939/Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia/LEPABE
info:eu-repo/grantAgreement/Comissão de Coordenação e Desenvolvimento Regional do Norte/P2020|Norte2020-Projetos Integrados ICDT/NORTE-01-0145-FEDER-000005/LEPABE-2-ECO-INNOVATION/LEPABE-2-ECO-INNOVATION
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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