Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/102630
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dc.creatorFilipa Castro
dc.creatorAntónio Ferreira
dc.creatorJosé A. Teixeira
dc.creatorFernando Rocha
dc.date.accessioned2019-08-13T23:08:42Z-
dc.date.available2019-08-13T23:08:42Z-
dc.date.issued2016
dc.identifier.issn1528-7483
dc.identifier.othersigarra:180050
dc.identifier.urihttps://hdl.handle.net/10216/102630-
dc.description.abstractIn the present work, it is reported for the first time the study of the applicability of a novel meso oscillatory flow reactor (meso-OFR) for protein crystallization as a process step. Crystallization assays carried out in the designed device enabled to derive a two-dimensional lysozyme phase diagram (lysozyme concentration against sodium chloride concentration). Results evidence the formation of several types of crystals (different size and shape), with a strong influence of salt concentration on crystal shape. Results also show that lysozyme remains active at the end of the experiments. Furthermore, it was possible to verify the reduction of the metastability zone when compared to lysozyme crystallization conducted under quiescent conditions. Induction times were also measured by online monitoring of the turbidity of the crystallization solution, obtained values being between 41 and 900 min. Beyond providing improved understanding of protein phase behavior under oscillatory flow mixing, the results are very promising regarding the feasibility of the designed methodology for protein crystallization as a process step.
dc.language.isoeng
dc.relationinfo:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Investigador FCT/IF/01087/2014/CP1249/CT0001/Projeto de investigação exploratória/IF/01087/2014/CP1249/CT0001
dc.relationinfo:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projetos Estratégicos/UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939/Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia/LEPABE
dc.relationinfo:eu-repo/grantAgreement/Comissão de Coordenação e Desenvolvimento Regional do Norte/P2020|Norte2020-Projetos Integrados ICDT/NORTE-01-0145-FEDER-000005/LEPABE-2-ECO-INNOVATION/LEPABE-2-ECO-INNOVATION
dc.rightsrestrictedAccess
dc.subjectEngenharia química, Engenharia química
dc.subjectChemical engineering, Chemical engineering
dc.titleProtein Crystallization As a Process Step in a Novel Meso Oscillatory Flow Reactor: Study of Lysozyme Phase Behavior
dc.typeArtigo em Revista Científica Internacional
dc.contributor.uportoFaculdade de Engenharia
dc.identifier.doi10.1021/acs.cgd.6b00262
dc.identifier.authenticusP-00K-P3Q
dc.subject.fosCiências da engenharia e tecnologias::Engenharia química
dc.subject.fosEngineering and technology::Chemical engineering
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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