Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/97481
Author(s): | Leal, IS Florido, M Andersen, P Appelberg, R |
Title: | Interleukin-6 regulates the phenotype of the immune response to a tuberculosis subunit vaccine |
Issue Date: | 2001 |
Abstract: | We investigated the role of interleukin-6 (IL-6) in the development of the immune response to a subunit vaccine against tuberculosis consisting of the culture filtrate proteins of Mycobacterium tuberculosis emulsified in the adjuvant dimethyldioctadecylammonium bromide (DDA). C57Bl/6 mice immunized with this vaccine developed a strong T helper 1 (Th1) response characterized by an increased production of interferon-gamma (IFN-gamma) secreted by CD4(+) T cells. Neutralization of IL-6 during in vivo priming resulted in marked reduction in the ability of T cells to secrete IFN-gamma and IL-2 and to proliferate. IL-6 gene-disrupted mice primed with the vaccine showed a decrease in the number of IFN-gamma -producing cells and an increase in IL-4-secreting cells as compared to control mice. In contrast, neutralization of IL-6 during a boost of the vaccine in previously primed mice did not affect the development of IFN-gamma -producing cells but still increased the number of IL-4-producing cells. Our work shows that IL-6 plays a major role in the priming but not in the later expression of a Th1 response to a tuberculosis vaccine. |
Subject: | Infecções, Medicina básica Infections, Basic medicine |
Scientific areas: | Ciências médicas e da saúde::Medicina básica Medical and Health sciences::Basic medicine |
URI: | https://hdl.handle.net/10216/97481 |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | restrictedAccess |
Appears in Collections: | ICBAS - Artigo em Revista Científica Internacional |
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88491. Restricted Access | 90.22 kB | Unknown | Request a copy from the Author(s) |
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