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https://hdl.handle.net/10216/95263
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DC Field | Value | Language |
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dc.creator | vitorino, r | |
dc.creator | guedes, sdm | |
dc.creator | ferreira, r | |
dc.creator | lobo, mjc | |
dc.creator | duarte, j | |
dc.creator | ferrer-correia, aj | |
dc.creator | tomer, kb | |
dc.creator | domingues, pm | |
dc.creator | amado, fml | |
dc.date.accessioned | 2022-12-08T00:22:47Z | - |
dc.date.available | 2022-12-08T00:22:47Z | - |
dc.date.issued | 2006 | |
dc.identifier.issn | 0909-8836 | |
dc.identifier.other | sigarra:45297 | |
dc.identifier.uri | https://hdl.handle.net/10216/95263 | - |
dc.description.abstract | In the present study, a proteomic approach was applied to evaluate the influence of salivary protein composition on in vitro dental pellicle formation and its possible correlation with dental caries. Whole saliva, collected from caries-free and caries-susceptible subjects, was analyzed by two-dimensional electrophoresis, and protein spots were identified by mass spectrometry. Data analysis of salivary protein composition showed a statistically significant correlation between the quantity of acidic proline-rich proteins (PRPs), lipocalin, cystatin SN and cystatin S, and samples from the caries-free group of subjects [decayed, missing or filled teeth (DMFT) = 0]. Samples from subjects with a high DMFT index appear to be correlated with high levels of amylase, immunoglobulin A, and lactoferrin. In vitro pellicle-composition experiments showed the same correlations found for whole saliva. As cystatins are known physiological inhibitors of cathepsins, the higher quantities of lipocalin, and cystatins S and SN found in the samples from the caries-free subjects suggest that inhibition of proteolytic events on other salivary proteins may indirectly provide tooth protection. The correlation between higher levels of the phosphorylated acidic PRPs 1/2 with samples from the caries-free group also suggests a protective role for these proteins. | |
dc.language.iso | eng | |
dc.rights | restrictedAccess | |
dc.subject | Medicina clínica | |
dc.subject | Clinical medicine | |
dc.title | Two-dimensional electrophoresis study of in vitro pellicle formation and dental caries susceptibility | |
dc.type | Artigo em Revista Científica Internacional | |
dc.contributor.uporto | Faculdade de Desporto | |
dc.identifier.doi | 10.1111/j.1600-0722.2006.00328.x | |
dc.identifier.authenticus | P-004-MDS | |
dc.subject.fos | Ciências médicas e da saúde::Medicina clínica | |
dc.subject.fos | Medical and Health sciences::Clinical medicine | |
Appears in Collections: | FADEUP - Artigo em Revista Científica Internacional |
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File | Description | Size | Format | |
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45297.pdf Restricted Access | 243.16 kB | Adobe PDF | Request a copy from the Author(s) |
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