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Author(s): Pedro Figueiredo
Scott K. Powers
Rita Ferreira
Hans J. Appell
J. A. Duarte
Title: Aging impairs skeletal muscle mitichondrial bioenergetic function
Issue Date: 2009
Abstract: This study investigated the infl uence of age on the functional status of mitochondria isolated from skeletal muscle of C57BL/6 mice aged 3 and 18 months. We hypothesized that skeletal muscle mitochondria isolated from aged animals will exhibit a decreased respiratory function. Mitochondrial respiratory functional measures (ie, State 3 and 4 respiration, respiratory control ratio and number of nanomoles of ADP phosphorylated by nanomoles of O 2 consumed per mitochondrion) and biochemical markers of oxidative damage (aconitase activity, protein carbonyl derivatives, sulfhydryl groups, and malondialdehyde ) were measured inisolated mitochondrial suspensions. Along with traditional tests of mitochondrial function, an in vitro repetitive ADP-stimulation test was used to evaluate the mitochondrial capacity to reestablish the homeostatic balance between successive ADP stimulations. The number of mitochondria per mitochondrial suspension, calculated by transmission electron microscopy, was used to normalize functional and biochemical data. Our results confi rm the existence of an age-associated decline in mitochondrial function of mixed skeletal muscle, which is signifi cantly correlated with higher levels of mitochondrial oxidative damage.
Subject: Fisiologia, Outras ciências médicas
Physiology, Other medical sciences
Scientific areas: Ciências médicas e da saúde::Outras ciências médicas
Medical and Health sciences::Other medical sciences
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FADEUP - Artigo em Revista Científica Internacional

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