Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/91278
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dc.creatorAngela França
dc.creatorVirgínia Carvalhais
dc.creatorManuel Vilanova
dc.creatorGerald B. Pier
dc.creatorNuno Cerca
dc.date.accessioned2019-03-05T00:07:00Z-
dc.date.available2019-03-05T00:07:00Z-
dc.date.issued2016
dc.identifier.issn2191-0855
dc.identifier.othersigarra:120941
dc.identifier.urihttps://hdl.handle.net/10216/91278-
dc.description.abstractBoth dynamic and fed-batch systems have been used for the study of biofilms. Dynamic systems, whose hallmark is the presence of continuous flow, have been considered the most appropriate for the study of the last stage of the biofilm lifecycle: biofilm disassembly. However, fed-batch is still the most used system in the biofilm research field. Hence, we have used a fed-batch system to collect cells released from Staphylococcus epidermidis biofilms, one of the most important etiological agents of medical device-associated biofilm infections. Herein, we showed that using this model it was possible to collect cells released from biofilms formed by 12 different S. epidermidis clinical and commensal isolates. In addition, our data indicated that biofilm disassembly occurred by both passive and active mechanisms, although the last occurred to a lesser extent. Moreover, it was observed that S. epidermidis biofilm-released cells presented higher tolerance to vancomycin and tetracycline, as well as a particular gene expression phenotype when compared with either biofilm or planktonic cells. Using this model, biofilm-released cells phenotype and their interaction with the host immune system could be studied in more detail, which could help providing significant insights into the pathophysiology of biofilm-related infections.
dc.language.isoeng
dc.rightsopenAccess
dc.titleCharacterization of an in vitro fed-batch model to obtain cells released from S. epidermidis biofilms
dc.typeArtigo em Revista Científica Internacional
dc.contributor.uportoInstituto de Ciências Biomédicas Abel Salazar
dc.identifier.doi10.1186/s13568-016-0197-9
dc.identifier.authenticusP-00K-AET
Appears in Collections:ICBAS - Artigo em Revista Científica Internacional

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