Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/53832
Author(s): Maiato, H
Rieder, Conly L
Swedlow, Jason R
Cole, Richard
Sunkel, CE
Earnshaw, William C
Title: Human CLASP1 is an outer kinetochore component that regulates spindle microtubule dynamics
Issue Date: 2003
Abstract: One of the most intriguing aspects of mitosis is the ability of kinetochores to hold onto the plus-ends of dynamic microtubules that are actively gaining or losing tubulin subunits. Here we show that the microtubule-associated protein CLASP1 is localized preferentially near the plus-ends of growing microtubules during spindle formation and is also a component of a novel region that we term the outer kinetochore corona. A truncated form of CLASP1 lacking the kinetochore-binding domain behaves as a dominant-negative, leading to the formation of unique single or double asters comprised of radial arrays of microtubule bundles that are highly resistant to depolymerization. Microinjection of cells with antibodies specific to CLASP1 causes bipolar spindles to collapse, forming bipolar or monopolar arrays of microtubules with chromosomes buried in the interior. Suppression of microtubule dynamics in injected cells rescues the kinetochore association with plus ends of microtubules at the periphery of the asters. Our data suggest that CLASP1 is required for kinetochore-associated microtubules to exhibit normal dynamic behaviour.
Subject: Ntibodies
Eukaryotic cells
HeLa Cells
Humans
Kinetochores
Microscopy
Electron
Microtubule-associated proteins
Microtubules
Mitosis
URI: http://hdl.handle.net/10216/53832
Catalogue Link: http://dx.doi.org/10.1016/S0092-8674(03)00465-3
Source: Cell, vol. 113(7), 891-904
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:I3S - Artigo em Revista Científica Internacional



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