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https://hdl.handle.net/10216/164833Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.creator | Batul Aliasger Qamri | |
| dc.date.accessioned | 2025-11-20T00:16:44Z | - |
| dc.date.available | 2025-11-20T00:16:44Z | - |
| dc.date.issued | 2024-11-19 | |
| dc.date.submitted | 2025-01-17 | |
| dc.identifier.other | sigarra:704206 | |
| dc.identifier.uri | https://hdl.handle.net/10216/164833 | - |
| dc.description | Introduction: Bone is a complex dynamic tissue that undergoes constant remodelling. Formation as well as repair of bone are nuanced complex procedures that are dependent not only the host factors, but also the local cellular factors. A cascade of events starting from the proliferation of mesenchymal and hematopoietic stem cells into osteoblasts, osteoclasts etc., to the formation of well a structured connective tissue, bone serves an important organ that provides structural as well as mechanical support to the body. Although bone with small defects is capable of healing itself, larger defects often require grafting procedures to restore its volume and quality. In order to regenerate bone, bone graft substitutes of different origins are employed, depending on the site and the nature of the defect. In the field of dental implantology specifically, bone regeneration is often required with concomitant implant placement to ensure optimal osteointegration of the implant. Implantology encompasses several aspects, such as biological, mechanical, morphological, and physicochemical considerations that serve as variables for predictable treatment outcomes. Within the realms of biological considerations, two important aspects of implantology include choice of materials of the implant and adequate bone volume for implant loading. Although titanium has been a long- standing gold standard as a choice of materials, limitations such as corrosion, metal allergies, poor aesthetics have led to the favour of zirconia implants. Since the interaction of implant with bone is crucial in the success of implant therapy, zirconia's osteoconductive potential must be harnessed to have predictable results. In order to maximize its potential, one such approach is to observe and assess the biological response of osteoblast-like cells, towards various bone grafts, cultured over zirconia. To further explore this, the present study aims to understand whether its presence alters the influence of various origin bone substitutes on osteoblast-like cells through an in vitro study model. Objectives: To evaluate the cellular metabolic activity and ALP expression of osteoblast-like cells in two ways: When indirectly exposed to various bone-graft substitutes (bovine origin, synthetic biphasic calcium phosphate and bioglass); when grown over zirconia substrates and placed in close proximity to the said bone graft substitutes and observe the differences. Materials and Methods: The presented study used a xenograft (Cerabone(r), Botiss, Germany), a synthetic biphasic calcium phosphate (Maxresorb(r), Botiss, Germany), and a synthetic bioactive glass (Novabone Dental Putty(r), Osteogenics, USA), along with custom-made zirconia discs (Patent(r), Zircon Medical Management AG, Germany). These biomaterials underwent surface characterization using SEM and EDS. Cytocompatibility assays were performed with osteoblast-like cells, evaluating their metabolic activity and alkaline phosphatase expression at defined time points. Two approaches were employed: first, cells were indirectly exposed to bone graft substitutes; and second, cells were cultured on zirconia and placed in close proximity to the bone graft substitutes. The results were analysed with one-way ANOVA, followed by the Tukey's post hoc (=0.05). Results and Discussion: With the indirect exposure to the bone graft substitutes, the metabolic assay of the cells revealed significantly lower values in the bioglass group on days 3 and 7, in comparison to control. The alkaline phosphatase activity revealed significantly lower values in xenograft and bioglass groups, on day 7, in comparison to control. Cells cultured over zirconia, when placed in close proximity to the bone grafts substitutes, revealed significantly lower metabolic activity on day 11 in all experimental groups, in comparison to control. The alkaline phosphatase activity revealed significantly higher values in bioglass group in comparison to control. The inverse results underscore how different bone graft substitutes can exert a varied influence on the cellular response and the presence of zirconia of can significantly alter the cellular response by modulating the cellular environment owing to its osteoconductive nature. Conclusions: Through this in vitro study, it can be concluded that bone grafts can have varying degrees of influence on cell proliferation and differentiation in the presence of zirconia materials. The results underscore interesting insights that could additionally implicate that the composition of bone graft substitutes plays a vital role in influencing cellular response in the presence of zirconia. | |
| dc.language.iso | eng | |
| dc.rights | openAccess | |
| dc.subject | Ciências médicas e da saúde | |
| dc.subject | Medical and Health sciences | |
| dc.title | Biocompatibility of hard tissue substitutes with zirconia: An in-vitro study to determine its influence on bone regeneration | |
| dc.type | Dissertação | |
| dc.contributor.uporto | Faculdade de Medicina Dentária | |
| dc.identifier.doi | 10.34626/wtn6-a455 | |
| dc.subject.fos | Ciências médicas e da saúde | |
| dc.subject.fos | Medical and Health sciences | |
| thesis.degree.discipline | Mestrado em Reabilitação Oral | |
| thesis.degree.grantor | Faculdade de Medicina Dentária | |
| thesis.degree.grantor | Universidade do Porto | |
| thesis.degree.level | 1 | |
| rcaap.embargofct | Possibilidade de publicação de artigo científico | |
| Appears in Collections: | FMDUP - Dissertação | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 704206.pdf | Biocompatibility of hard tissue substitutes with zirconia: An in-vitro study to determine its influence on bone regeneration | 3.58 MB | Adobe PDF |  View/Open |
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