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Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/158018
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dc.creatorCosta, S-
dc.creatorPires, J-
dc.creatorTeixeira-Gomes, A-
dc.date.accessioned2024-04-12T13:39:27Z-
dc.date.available2024-04-12T13:39:27Z-
dc.date.issued2023-
dc.identifier.isbn978-0-323-85434-4-
dc.identifier.urihttps://hdl.handle.net/10216/158018-
dc.description.abstractThe comet assay is a sensitive, inexpensive, fast, and easy-to-use method widely used in genotoxicity testing and biomonitoring studies. It can be applied using a few cells and on almost every cell type to assess a range of different DNA lesions, DNA repair activity and even DNA methylation levels, at the individual cell level. The principle is simple, during electrophoresis relaxed DNA strands move toward the anode away from the nucleoid core, appearing like a “comet”, hence the name of the assay. Incubation with lesion specific enzymes allows the detection of specific types of DNA lesions such as DNA oxidative damage, repair efficiency and methylation. The distribution of DNA within the comet relates to the extent of DNA damage present in the cell. © 2024 Elsevier Inc. All rights reserved.pt_PT
dc.description.sponsorshipArmanda Teixeira-Gomes and Joana Pires are supported by Fundação para a Ciência e Tecnologia (FCT-MCTES), and by the European Social Fund, through Programa Operacional Capital Humano (POCH), under the individual grant SFRH/BD/121802/2016 and project grant PCIF/SSO/0017/2018.pt_PT
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/BD/121802/2016/PTpt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/Concurso de Projetos de Investigação Científica e Desenvolvimento Tecnológico no Âmbito da Prevenção e Combate a Incêndios Florestais - 2018/PCIF/SSO/0017/2018/PTpt_PT
dc.relation.ispartofEncyclopedia of Toxicology (Fourth Edition)-
dc.rightsrestrictedAccesspt_PT
dc.titleComet assaypt_PT
dc.typeCapítulo ou Parte de Livropt_PT
dc.contributor.uportoInstituto de Saúde Públicapt_PT
dc.identifier.doi10.1016/B978-0-12-824315-2.01072-1-
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/abs/pii/B9780128243152010721?via%3Dihub-
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