Avian Malaria in wild birds from a wildlife rehabilitation center in Central Portugal

Abstract

Avian haemosporidian (Haemoproteus, Leucocytozoon, Plasmodium) are vector-transmitted protozoan parasites highly prevalent in various bird species. Still, their importance for bird health, species decline, or impact on rehabilitation success is underestimated. This study aimed to determine the occurrence and diversity of haemosporidian parasites after necropsies of seventy wild birds from thirty-four species of twelve taxonomic orders. Detection of avian haemosporidian DNA was evaluated using PCR amplification of the cytochrome b gene. 48.6% of all sampled birds were positive, with 24.3% positive for Plasmodium spp./Haemoproteus spp. and 44.3% for Leucocytozoon spp. Mixed infections corresponded to 20% of all tested birds. Sequencing of several selected samples revealed the infection of Plasmodium matutinum, Plasmodium relictum and different lineages of Leucocytozoon spp. This study provides a baseline description of haemosporidian infections in wild birds from a rehabilitation center in central Portugal. The results show the necessity to test and monitor possible infections that undermine recovery processes for different birds. Further research into the occurrence of these haemosporidian species in birds kept in conservation centers is needed to understand the impact on bird health.