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Author(s): da Silva, PG
Gonçalves, J
Lopes, AIB
Esteves, NA
Bamba, GEE
Nascimento, MSJ
Branco, PTBS
Soares, RRG
Sousa, SIV
Mesquita, JR
Title: Evidence of Air and Surface Contamination with SARS-CoV-2 in a Major Hospital in Portugal
Publisher: MDPI
Issue Date: 2022
Abstract: As the third wave of the COVID-19 pandemic hit Portugal, it forced the country to reintro-duce lockdown measures due to hospitals reaching their full capacities. Under these circumstances, environmental contamination by SARS-CoV-2 in different areas of one of Portugal’s major Hospitals was assessed between 21 January and 11 February 2021. Air samples (n = 44) were collected from eleven different areas of the Hospital (four COVID-19 and seven non-COVID-19 areas) using Coriolis® µ and Coriolis® Compact cyclone air sampling devices. Surface sampling was also performed (n = 17) on four areas (one COVID-19 and three non-COVID-19 areas). RNA extraction followed by a one-step RT-qPCR adapted for quantitative purposes were performed. Of the 44 air samples, two were positive for SARS-CoV-2 RNA (6575 copies/m3 and 6662.5 copies/m3, respectively). Of the 17 surface samples, three were positive for SARS-CoV-2 RNA (200.6 copies/cm2, 179.2 copies/cm2, and 201.7 copies/cm2, respectively). SARS-CoV-2 environmental contamination was found both in air and on surfaces in both COVID-19 and non-COVID-19 areas. Moreover, our results suggest that longer collection sessions are needed to detect point contaminations. This reinforces the need to remain cautious at all times, not only when in close contact with infected individuals. Hand hygiene and other standard transmission-prevention guidelines should be continuously followed to avoid nosocomial COVID-19. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Subject: Air samples; Environmental contamination; SARS-CoV-2; Surface samples
Source: Int J Environ Res Public Health. 2022 Jan 4;19(1):525. doi: 10.3390/ijerph19010525.
Related Information: info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB/00511/2020/PT
info:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND/02477/2017/CP1399/CT0007/PT
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:ISPUP - Artigo em Revista Científica Internacional

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