Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/145275
Author(s): | Amorim, JP Bordeira-Carriço, R Gali-Macedo, A Perrod, C Bessa, J |
Title: | CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish |
Publisher: | Cell Press |
Issue Date: | 2020 |
Abstract: | Since its first application for site-directed mutagenesis, the CRISPR-Cas9 system has revolutionized genome engineering. Here, we present a validated workflow for the generation of targeted genomic deletions in zebrafish, including the design, cloning, and synthesis of single-guide RNAs and Cas9 mRNA, followed by microinjection in zebrafish embryos and subsequent genotype screening for the establishment of a mutant line. The versatility and efficiency of this pipeline makes the generation of zebrafish models a widely used approach in functional genetics. For complete details on the use and execution of this protocol, please refer to Amorim et al. (2020). |
Subject: | CRISPR Genetics Genomics Model Organisms |
URI: | https://hdl.handle.net/10216/145275 |
Source: | STAR Protocols, vol.1(3):100208 |
Related Information: | info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F145110%2F2019/PT |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | openAccess |
License: | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
Appears in Collections: | I3S - Artigo em Revista Científica Internacional |
Files in This Item:
File | Description | Size | Format | |
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10.1016-j.xpro.2020.100208.pdf | 4.32 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License