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Author(s): Amorim, JP
Bordeira-Carriço, R
Gali-Macedo, A
Perrod, C
Bessa, J
Title: CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish
Publisher: Cell Press
Issue Date: 2020
Abstract: Since its first application for site-directed mutagenesis, the CRISPR-Cas9 system has revolutionized genome engineering. Here, we present a validated workflow for the generation of targeted genomic deletions in zebrafish, including the design, cloning, and synthesis of single-guide RNAs and Cas9 mRNA, followed by microinjection in zebrafish embryos and subsequent genotype screening for the establishment of a mutant line. The versatility and efficiency of this pipeline makes the generation of zebrafish models a widely used approach in functional genetics. For complete details on the use and execution of this protocol, please refer to Amorim et al. (2020).
Subject: CRISPR
Model Organisms
Source: STAR Protocols, vol.1(3):100208
Related Information: info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F145110%2F2019/PT
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:I3S - Artigo em Revista Científica Internacional

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