1. Repositório Aberto da Universidade do Porto
  2. I3S - Instituto de Investigação e Inovação em Saúde
  3. I3S - Artigo em Revista Científica Internacional
Utilize este identificador para referenciar este registo: https://hdl.handle.net/10216/145266
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Campo DCValorIdioma
dc.creatorSocodato, R
dc.creatorMelo, PN
dc.creatorFerraz-Nogueira, JP
dc.creatorPortugal, CC
dc.creatorRelvas, JB
dc.date.accessioned2022-11-17T11:33:08Z-
dc.date.available2022-11-17T11:33:08Z-
dc.date.issued2020
dc.identifier.issn2666-1667
dc.identifier.urihttps://hdl.handle.net/10216/145266-
dc.description.abstractThis protocol highlights the use of FRET-based biosensors to investigate signaling events during microglia activation in real time. Understanding microglia activation has gained momentum as it can help decipher signaling mechanisms underlying the neurodegenerative process occurring in neurological disorders. Unlike more traditional methods widely employed in the microglia field, FRET allows microglia signaling events to be studied in real time with exquisite subcellular resolution. However, FRET-based live-cell imaging requires application-specific biosensors and specialized imaging systems, limiting its use in in vivo studies. For complete details on the use and execution of this protocol, please refer to Socodato et al. (2020), Portugal et al. (2017), and Socodato et al. (2018).
dc.description.sponsorshipThis work was financed by FEDER (Fundo Europeu de Desenvolvimento Regional) funds through the COMPETE 2020 - Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, and by Portuguese funds through FCT (Fundação para a Ciência e a Tecnologia)/Ministério da Ciência, Tecnologia e Ensino Superior in the framework of the project POCI-01-0145-FEDER-031318 (PTDC/MED-NEU/31318/2017). The authors acknowledge the support of the following: i3S Scientific Platform: Advanced Light Microscopy (ALM), members of the national infrastructure PPBI-Portuguese Platform of BioImaging (supported by POCI-01–0145-FEDER-022122). C.C.P. and R.S. hold employment contracts financed by national funds through FCT – Fundação para a Ciência e a Tecnologia, IP, in the context of the program-contract described in paragraphs 4, 5, and 6 of art. 23 of Law no. 57/2016, of August 29th, as amended by Law no. 57/2017 of July 19th.
dc.language.isoeng
dc.publisherCell Press
dc.relationinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FMED-NEU%2F31318%2F2017/PT
dc.relation.ispartofSTAR Protocols, vol.1(3):100147
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.meshBiosensing Techniques / methods
dc.subject.meshCell Line
dc.subject.meshDiagnostic Imaging
dc.subject.meshFluorescence Resonance Energy Transfer / methods
dc.subject.meshFluorescent Antibody Technique / methods
dc.subject.meshMicroglia / cytology
dc.subject.meshMicroglia / metabolism
dc.subject.meshMicroglia / physiology
dc.subject.meshMicroscopy, Fluorescence / methods
dc.subject.meshSignal Transduction
dc.subject.meshStaining and Labeling / methods
dc.titleA Protocol for FRET-Based Live-Cell Imaging in Microglia
dc.typeArtigo em Revista Científica Internacional
dc.contributor.uportoInstituto de Investigação e Inovação em Saúde
dc.identifier.doi10.1016/j.xpro.2020.100147
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S2666166720301349?via%3Dihub
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