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dc.creatorAlbuquerque, A
dc.creatorFernandes, M
dc.creatorStirrup, O
dc.creatorTeixeira, AL
dc.creatorSantos, J
dc.creatorRodrigues, M
dc.creatorRios, E
dc.creatorMacedo, G
dc.creatorMedeiros, R
dc.description.abstractAnal squamous intraepithelial lesions (ASIL) or anal intraepithelial neoplasia (AIN) are precancerous lesions. microRNAs (miRNAs) have been implicated in cervical carcinogenesis, but have never been assessed in anal precancerous lesions. Our aim was to evaluate the expression of miR-16, miR-20a, miR-150 and miR-155 in several grades of ASIL obtained from high-risk patients, submitted to anal cancer screening from July 2016 to January 2017. Lesions were classified according to the Lower Anogenital Squamous Terminology (LAST) in low-grade (LSIL) and high-grade squamous intraepithelial lesions (HSIL), and the AIN classification in AIN1, AIN2 and AIN3. A hundred and five biopsies were obtained from 60 patients. Ten samples were negative (9.5%), 63 were LSIL (60%) and 32 were HSIL (30.5%) according to the LAST. Twenty seven (26%) were negative for dysplasia, 46 were classified as AIN1 (44%), 14 as AIN2 (13%) and 18 as AIN3 (17%) according to the AIN classification. There was no statistically significant difference in the fold expression of miR-16, miR-20a, miR-150 and miR-155, according to either classification. Although non- significant, there was an increasing trend in the miR-155 fold expression from negative samples to HSIL, with the highest fold expression increase in both LSIL and HSIL compared to the other miRNAs.
dc.publisherNature Publishing Group
dc.relation.ispartofScientific Reports, vol.9(1):1523
dc.subject.meshAnus Neoplasms / genetics
dc.subject.meshAnus Neoplasms / pathology
dc.subject.meshAnus Neoplasms / virology
dc.subject.meshBiomarkers, Tumor / genetics
dc.subject.meshCarcinoma in Situ / genetics
dc.subject.meshCarcinoma in Situ / pathology
dc.subject.meshCarcinoma in Situ / virology
dc.subject.meshCase-Control Studies
dc.subject.meshMicroRNAs / genetics
dc.subject.meshPapillomaviridae / isolation & purification
dc.subject.meshPapillomavirus Infections / complications
dc.subject.meshPapillomavirus Infections / pathology
dc.subject.meshPapillomavirus Infections / virology
dc.subject.meshPrecancerous Conditions / genetics
dc.subject.meshPrecancerous Conditions / pathology
dc.subject.meshPrecancerous Conditions / virology
dc.subject.meshRisk Factors
dc.subject.meshSquamous Intraepithelial Lesions / genetics
dc.subject.meshSquamous Intraepithelial Lesions / pathology
dc.subject.meshSquamous Intraepithelial Lesions / virology
dc.titleExpression of microRNAs 16, 20a, 150 and 155 in anal squamous intraepithelial lesions from high-risk groups
dc.typeArtigo em Revista Científica Internacional
dc.contributor.uportoInstituto de Investigação e Inovação em Saúde
Appears in Collections:I3S - Artigo em Revista Científica Internacional

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