Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/136252
Author(s): Afonso, O
Castellani, CM
Cheeseman, LP
Ferreira, JG
Orr, B
Ferreira, LT
Chambers, JJ
Morais-de-Sá, E
Maresca, TJ
Maiato, H
Title: Spatiotemporal control of mitotic exit during anaphase by an aurora B-Cdk1 crosstalk
Publisher: eLife Sciences Publications
Issue Date: 2019
Abstract: According to the prevailing ‘clock’ model, chromosome decondensation and nuclear envelope reformation when cells exit mitosis are byproducts of Cdk1 inactivation at the metaphase-anaphase transition, controlled by the spindle assembly checkpoint. However, mitotic exit was recently shown to be a function of chromosome separation during anaphase, assisted by a midzone Aurora B phosphorylation gradient-the ‘ruler’ model. Here we found that Cdk1 remains active during anaphase due to ongoing APC/CCdc20- and APC/CCdh1-mediated degradation of B-type Cyclins in Drosophila and human cells. Failure to degrade B-type Cyclins during anaphase prevented mitotic exit in a Cdk1-dependent manner. Cyclin B1-Cdk1 localized at the spindle midzone in an Aurora B-dependent manner, with incompletely separated chromosomes showing the highest Cdk1 activity. Slowing down anaphase chromosome motion delayed Cyclin B1 degradation and mitotic exit in an Aurora B-dependent manner. Thus, a crosstalk between molecular ‘rulers’ and ‘clocks’ licenses mitotic exit only after proper chromosome separation.
Subject: Aurora B
Cdk1
Cyclin B1
D. melanogaster
anaphase
cell biology
human
mitosis
mitotic exit
mouse
URI: https://hdl.handle.net/10216/136252
Source: eLife, vol.8:e47646
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
License: https://creativecommons.org/licenses/by/4.0/
Appears in Collections:I3S - Artigo em Revista Científica Internacional

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