Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/136252
Author(s): | Afonso, O Castellani, CM Cheeseman, LP Ferreira, JG Orr, B Ferreira, LT Chambers, JJ Morais-de-Sá, E Maresca, TJ Maiato, H |
Title: | Spatiotemporal control of mitotic exit during anaphase by an aurora B-Cdk1 crosstalk |
Publisher: | eLife Sciences Publications |
Issue Date: | 2019 |
Abstract: | According to the prevailing ‘clock’ model, chromosome decondensation and nuclear envelope reformation when cells exit mitosis are byproducts of Cdk1 inactivation at the metaphase-anaphase transition, controlled by the spindle assembly checkpoint. However, mitotic exit was recently shown to be a function of chromosome separation during anaphase, assisted by a midzone Aurora B phosphorylation gradient-the ‘ruler’ model. Here we found that Cdk1 remains active during anaphase due to ongoing APC/CCdc20- and APC/CCdh1-mediated degradation of B-type Cyclins in Drosophila and human cells. Failure to degrade B-type Cyclins during anaphase prevented mitotic exit in a Cdk1-dependent manner. Cyclin B1-Cdk1 localized at the spindle midzone in an Aurora B-dependent manner, with incompletely separated chromosomes showing the highest Cdk1 activity. Slowing down anaphase chromosome motion delayed Cyclin B1 degradation and mitotic exit in an Aurora B-dependent manner. Thus, a crosstalk between molecular ‘rulers’ and ‘clocks’ licenses mitotic exit only after proper chromosome separation. |
Subject: | Aurora B Cdk1 Cyclin B1 D. melanogaster anaphase cell biology human mitosis mitotic exit mouse |
URI: | https://hdl.handle.net/10216/136252 |
Source: | eLife, vol.8:e47646 |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | openAccess |
License: | https://creativecommons.org/licenses/by/4.0/ |
Appears in Collections: | I3S - Artigo em Revista Científica Internacional |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
10.7554-eLife.47646.pdf | 30.27 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License