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Author(s): Wiegand, S
Jogler, M
Boedeker, C
Pinto, D
Vollmers, J
Rivas-Marín, E
Kohn, T
Peeters, S.H
Heuer, A
Rast, P
Oberbeckmann, S
Bunk, B
Jeske, O
Meyerdierks, A
Storesund, J.E
Kallscheuer, N
Lücker, S
Lage, O.M
Pohl, T
Merkel, B.J
Hornburger, P
Müller, R.W
Brümmer, F
Labrenz, M
Spormann, A.M
Op den Camp, H.J.M
Overmann, J
Amann, R
Jetten, M.S.M
Mascher, T
Medema, M.H
Devos, D.P
Kaster, A.K
Øvreås, L
Rohde, M
Galperin, M.Y
Jogler, C.
Title: Cultivation and functional characterization of 79 planctomycetes uncovers their unique biology
Publisher: Nature Microbiology
Issue Date: 2019
Abstract: When it comes to the discovery and analysis of yet uncharted bacterial traits, pure cultures are essential as only these allow detailed morphological and physiological characterization as well as genetic manipulation. However, microbiologists are struggling to isolate and maintain the majority of bacterial strains, as mimicking their native environmental niches adequately can be a challenging task. Here, we report the diversity-driven cultivation, characterization and genome sequencing of 79 bacterial strains from all major taxonomic clades of the conspicuous bacterial phylum Planctomycetes. The samples were derived from different aquatic environments but close relatives could be isolated from geographically distinct regions and structurally diverse habitats, implying that ‘everything is everywhere’. With the discovery of lateral budding in ‘Kolteria novifilia’ and the capability of the members of the Saltatorellus clade to divide by binary fission as well as budding, we identified previously unknown modes of bacterial cell division. Alongside unobserved aspects of cell signalling and small-molecule production, our findings demonstrate that exploration beyond the well-established model organisms has the potential to increase our knowledge of bacterial diversity. We illustrate how ‘microbial dark matter’ can be accessed by cultivation techniques, expanding the organismic background for small-molecule research and drug-target detection. © 2019, The Author(s), under exclusive licence to Springer Nature Limited.
Subject: amino acid analysis
aquatic environment
bacterial cell
bacterial genome
bacterial strain
bacterial structures
bacterium culture
bacterium identification
cell division
cell structure
electron microscopy
gene sequence
genetic analysis
microbial diversity
priority journal
signal transduction
species habitat
bacterial phenomena and functions
genetic variation
growth, development and aging
secondary metabolism
Bacterial Physiological Phenomena
Cell Division
Genetic Variation
Genome, Bacterial
RNA, Ribosomal, 16S
Secondary Metabolism
Signal Transduction
Source: Nature Microbiology volume 5, pages126–140(2020)
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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