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Author(s): Rego, A.
Sousa, A.G.G.
Santos, J.P.
Pascoal, F.
Canário, J.
Leão, P.N.
Magalhães, C.
Title: Diversity of bacterial biosynthetic genes in maritime antarctica
Publisher: MDPI
Issue Date: 2020
Abstract: Bacterial natural products (NPs) are still a major source of new drug leads. Polyketides (PKs) and non-ribosomal peptides (NRP) are two pharmaceutically important families of NPs and recent studies have revealed Antarctica to harbor endemic polyketide synthase (PKS) and non-ribosomal peptide synthetase (NRPS) genes, likely to be involved in the production of novel metabolites. Despite this, the diversity of secondary metabolites genes in Antarctica is still poorly explored. In this study, a computational bioprospection approach was employed to study the diversity and identity of PKS and NRPS genes to one of the most biodiverse areas in maritime Antarctica—Maxwell Bay. Amplicon sequencing of soil samples targeting ketosynthase (KS) and adenylation (AD) domains of PKS and NRPS genes, respectively, revealed abundant and unexplored chemical diversity in this peninsula. About 20% of AD domain sequences were only distantly related to characterized biosynthetic genes. Several PKS and NRPS genes were found to be closely associated to recently described metabolites including those from uncultured and candidate phyla. The combination of new approaches in computational biology and new culture-dependent and -independent strategies is thus critical for the recovery of the potential novel chemistry encoded in Antarctica microorganisms.
Subject: Adenylation (AD)
Biosynthetic genes
Computational bioprospection
Ketosynthase (KS)
Natural products (NPs)
Non-ribosomal peptides (NRPs)
Polyketides (PKs)
Source: Microorganisms 2020, 8(2), 279
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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