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Author(s): Lopes, C
Gomes, C
Neto, E
Sampaio, P
Aguiar, P
Pêgo, AP
Title: Microfluidic-based platform to mimic the in vivo peripheral administration of neurotropic nanoparticles
Publisher: Future Medicine
Issue Date: 2016
Abstract: Aim: Propose a nanoparticle for neuron-targeted retrograde gene delivery and describe a microfluidic-based culture system to provide insight into vector performance and safety. Methods: Using compartmentalized neuron cultures we dissected nanoparticle bioactivity upon delivery taking advantage of (quantitative) bioimaging tools. Results: Targeted and nontargeted nanoparticles were internalized at axon terminals and retrogradely transported to cell bodies at similar average velocities but the former have shown an axonal flux 2.7-times superior to nontargeted nanoparticles, suggesting an improved cargo-transportation efficiency. The peripheral administration of nanoparticles to axon terminals is nontoxic as compared with their direct administration to the cell body or whole neuron. Conclusion: A neuron-targeted nanoparticle system was put forward. Microfluidic-based neuron cultures are proposed as a powerful tool to investigate nanoparticle bio-performance.
Subject: gene delivery
targeted nanoparticles
Source: Nanomedicine, vol. 11(24), p. 3205-3221
Related Information: info:eu-repo/grantAgreement/FCT/5876-PPCDTI/115124/PT
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:I3S - Artigo em Revista Científica Internacional

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