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Author(s): Rocha, D
Brites, P
Fonseca, C
Pêgo, AP
Title: Poly(trimethylene carbonate-co-e-caprolactone) promotes axonal growth
Publisher: Public Library of Science
Issue Date: 2014
Abstract: Mammalian central nervous system (CNS) neurons do not regenerate after injury due to the inhibitory environment formed by the glial scar, largely constituted by myelin debris. The use of biomaterials to bridge the lesion area and the creation of an environment favoring axonal regeneration is an appealing approach, currently under investigation. This work aimed at assessing the suitability of three candidate polymers - poly(e-caprolactone), poly(trimethylene carbonate-co-e-caprolactone) (P(TMC-CL)) (11:89 mol%) and poly(trimethylene carbonate) - with the final goal of using these materials in the development of conduits to promote spinal cord regeneration. Poly(L-lysine) (PLL) coated polymeric films were tested for neuronal cell adhesion and neurite outgrowth. At similar PLL film area coverage conditions, neuronal polarization and axonal elongation was significantly higher on P(TMC-CL) films. Furthermore, cortical neurons cultured on P(TMC-CL) were able to extend neurites even when seeded onto myelin. This effect was found to be mediated by the glycogen synthase kinase 3ß (GSK3ß) signaling pathway with impact on the collapsin response mediator protein 4 (CRMP4), suggesting that besides surface topography, nanomechanical properties were implicated in this process. The obtained results indicate P(TMC-CL) as a promising material for CNS regenerativ e applications as it promotes axonal growth, overcoming myelin inhibition.
Subject: Animals
Axons/drug effects
Cell Adhesion/drug effects
Cerebral Cortex/cytology
Cerebral Cortex/metabolism
Glycogen Synthase Kinase 3/metabolism
Glycogen Synthase Kinase 3 beta
Myelin Sheath/metabolism
Neurites/drug effects
Neurons/drug effects
Signal Transduction/drug effects
Source: PLoS ONE, vol. 9(2):e88593
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:I3S - Artigo em Revista Científica Internacional

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