Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120726
Author(s): Pires, L
Guarino, V
Oliveira, M
Ribeiro, C
Barbosa, M
Ambrosio, L
Pêgo, AP
Title: Ibuprofen-loaded poly(trimethylene carbonate-co-e-caprolactone) electrospun fibres for nerve regeneration
Publisher: John Wiley and Sons
Issue Date: 2016
Abstract: The development of scaffolds that combine the delivery of drugs with the physical support provided by electrospun fibres holds great potential in the field of nerve regeneration. Here it is proposed the incorporation of ibuprofen, a well-known non-steroidal anti-inflammatory drug, in electrospun fibres of the statistical copolymer poly(trimethylene carbonate-co-e-caprolactone) [P(TMC-CL)] to serve as a drug delivery system to enhance axonal regeneration in the context of a spinal cord lesion, by limiting the inflammatory response. P(TMC-CL) fibres were electrospun from mixtures of dichloromethane (DCM) and dimethylformamide (DMF). The solvent mixture applied influenced fibre morphology, as well as mean fibre diameter, which decreased as the DMF content in solution increased. Ibuprofen-loaded fibres were prepared from P(TMC-CL) solutions containing 5% ibuprofen (w/w of polymer). Increasing drug content to 10% led to jet instability, resulting in the formation of a less homogeneous fibrous mesh. Under the optimized conditions, drug-loading efficiency was above 80%. Confocal Raman mapping showed no preferential distribution of ibuprofen in P(TMC-CL) fibres. Under physiological conditions ibuprofen was released in 24h. The release process being diffusion-dependent for fibres prepared from DCM solutions, in contrast to fibres prepared from DCM-DMF mixtures where burst release occurred. The biological activity of the drug released was demonstrated using human-derived macrophages. The release of prostaglandin E 2 to the cell culture medium was reduced when cells were incubated with ibuprofen-loaded P(TMC-CL) fibres, confirming the biological significance of the drug delivery strategy presented. Overall, this study constitutes an important contribution to the design of a P(TMC-CL)-based nerve conduit with anti-inflammatory properties.
Subject: Confocal Raman microscopy
Drug delivery
Electrospinning
Ibuprofen
Inflammation
Nerve guide
URI: https://hdl.handle.net/10216/120726
Source: Journal of Tissue Engineering and Regenerative Medicine, vol. 10(3), p. E154-E166
Related Information: info:eu-repo/grantAgreement/FCT/5876-PPCDTI/115124/PT
info:eu-repo/grantAgreement/FCT/5876-PPCDTI/112511/PT
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
License: https://creativecommons.org/licenses/by-nc/4.0/
Appears in Collections:I3S - Artigo em Revista Científica Internacional

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