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Author(s): Mereiter, S
Macedo, JA
Polom, K
Roviello, F
Magalhães, A
Reis, CA
Title: O-glycan truncation enhances cancer-related functions of CD44 in gastric cancer.
Publisher: John Wiley & Sons
Issue Date: 2019-05-11
Abstract: CD44 isoforms are often upregulated in gastric cancer and have been associated with increased metastatic potential and poor survival. To evaluate the functional impact of O-glycan truncation on CD44 we have analysed glyco-engineered cancer cell models displaying shortened O-glycans. Here, we demonstrate that induction of aberrant O-glycan termination through various molecular mechanisms affects CD44 molecular features. We show that CD44 is a major carrier of truncated O-glycans and that this truncation is accompanied by an increased hyaluronan binding capacity and affects extracellular shedding. In addition, short O-glycans promoted the colocalization of CD44v6 with the receptor tyrosine kinase RON and concomitantly increased activation. Our in vitro findings were validated in gastric cancer clinical samples.
Subject: CD44
Gastric cancer
Hyaluronic acid
Proximity ligation assay
Series: FEBS letters doi: 10.1002/1873-3468.13432
Related Information: info:eu-repo/grantAgreement/FCT/5876/147342/PT
Document Type: Artigo em Revista Científica Internacional
Rights: embargoedAccess
Embargo End Date: 2020-05-11
Appears in Collections:I3S - Artigo em Revista Científica Internacional

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