Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/120535
Author(s): | Alexandrino D.A.M. Ribeiro I. Pinto L.M. Cambra R. Oliveira R.S. Pereira F. Carvalho M.F. |
Title: | Biodegradation of mono-, di- and trifluoroacetate by microbial cultures with different origins |
Publisher: | Elsevier |
Issue Date: | 2018 |
Abstract: | This work focused on the biodegradation of three structurally related fluoroacetates (FAs), mono- (MFA), di- (DFA) and trifluoroacetate (TFA), using as microbial inocula samples collected from a site with a long history of industrial contamination and activated sludge obtained from a municipal wastewater treatment plant. Biodegradation experiments were carried out under different modes of substrate supplementation, which included (i) FAs fed as sole carbon sources; (ii) FAs (only for DFA and TFA) fed in co-metabolism with sodium acetate; and (iii) mixtures of MFA with DFA or TFA. Biodegradation of the target compounds was assessed through fluoride ion release. Defluorination was obtained in the cultures fed with MFA, while DFA and TFA were recalcitrant in all tested conditions. When present in mixture, DFA was shown to inhibit biodegradation of MFA, while TFA had no effect. A total of 13 bacterial isolates obtained from MFA degrading cultures were found to degrade 20 mgL −1 of this compound, as single strains, when supplemented as a sole carbon source. Sequencing of the 16S rRNA gene indicated that among these degrading bacteria only Delftia acidovorans had been previously reported to be able to degrade MFA. This work shows that, despite their similar chemical structures, biodegradation of the three tested FAs is very distinct and draws attention to the unknown impacts that the accumulation of DFA and TFA may have in the environment as a result of their high recalcitrance. © 2017 Elsevier |
Subject: | Activated sludge process Carbon Fluorine compounds Industrial water treatment Metabolism Mixtures Physiology RNA Sodium compounds Wastewater treatment Co metabolisms Defluorination Difluoroacetate Monofluoroacetate Trifluoroacetates Biodegradation acetic acid bacterial RNA difluoroacetate fluoride ion fluoroacetic acid monofluoroacetate RNA 16S trifluoroacetic acid unclassified drug fluoroacetic acid Achromobacter Achromobacter anxifer activated sludge aerobic metabolism Arthrobacter Arthrobacter humicola Article bacterial growth bacterial strain bacterium culture bacterium isolate Chryseobacterium Chryseobacterium taeanense Comamonas testosteroni controlled study defluorination Delftia acidovorans gene sequence Herbaspirillum Herbaspirillum frisingense industrial waste microbial consortium microbial degradation municipal solid waste nonhuman nucleotide sequence organisms by carbon source Phragmites australis priority journal Pseudomonas putida rhizosphere inoculation sediment Stenotrophomonas maltophilia Variovorax Variovorax paradoxus waste water treatment plant bioremediation Delftia isolation and purification metabolism Biodegradation, Environmental Delftia Fluoroacetates |
URI: | https://hdl.handle.net/10216/120535 |
Source: | New Biotechnology, vol. 43, p. 23-29 |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | restrictedAccess |
Appears in Collections: | CIIMAR - Artigo em Revista Científica Internacional |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Alexandrino DAM_2018.pdf Restricted Access | 346.62 kB | Adobe PDF | Request a copy |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.