Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120518
Author(s): Barbosa C.S.
da Fonseca R.R.
Batista T.M.
Barreto M.A.
Argolo C.S.
de Carvalho M.R.
do Amaral D.O.J.
Silva E.M.A.
Arévalo-Gardini E.
Hidalgo K.S.
Franco G.R.
Pirovani C.P.
Micheli F.
Gramacho K.P.
Title: Genome sequence and effectorome of Moniliophthora perniciosa and Moniliophthora roreri subpopulations
Publisher: BMC
Issue Date: 2018
Abstract: Background: The hemibiotrophic pathogens Moniliophthora perniciosa (witches' broom disease) and Moniliophthora roreri (frosty pod rot disease) are among the most important pathogens of cacao. Moniliophthora perniciosa has a broad host range and infects a variety of meristematic tissues in cacao plants, whereas M. roreri infects only pods of Theobroma and Herrania genera. Comparative pathogenomics of these fungi is essential to understand Moniliophthora infection strategies, therefore the detection and in silico functional characterization of effector candidates are important steps to gain insight on their pathogenicity. Results: Candidate secreted effector proteins repertoire were predicted using the genomes of five representative isolates of M. perniciosa subpopulations (three from cacao and two from solanaceous hosts), and one representative isolate of M. roreri from Peru. Many putative effectors candidates were identified in M. perniciosa: 157 and 134 in cacao isolates from Bahia, Brazil; 109 in cacao isolate from Ecuador, 92 and 80 in wild solanaceous isolates from Minas Gerais (Lobeira) and Bahia (Caiçara), Brazil; respectively. Moniliophthora roreri showed the highest number of effector candidates, a total of 243. A set of eight core effectors were shared among all Moniliophthora isolates, while others were shared either between the wild solanaceous isolates or among cacao isolates. Mostly, candidate effectors of M. perniciosa were shared among the isolates, whereas in M. roreri nearly 50% were exclusive to the specie. In addition, a large number of cell wall-degrading enzymes characteristic of hemibiotrophic fungi were found. From these, we highlighted the proteins involved in cell wall modification, an enzymatic arsenal that allows the plant pathogens to inhabit environments with oxidative stress, which promotes degradation of plant compounds and facilitates infection. Conclusions: The present work reports six genomes and provides a database of the putative effectorome of Moniliophthora, a first step towards the understanding of the functional basis of fungal pathogenicity. © 2018 The Author(s).
Subject: effectorome
proteome
unclassified drug
fungal DNA
fungal protein
Article
Brazil
cacao
controlled study
Ecuador
fungal cell wall
fungal genome
fungal virulence
gene sequence
Moniliophthora
Moniliophthora perniciosa
Moniliophthora roreri
nonhuman
oxidative stress
Peru
Solanaceae
Agaricales
chemistry
classification
genetics
isolation and purification
metabolism
microbiology
phylogeny
plant disease
whole genome sequencing
Agaricales
Brazil
Cacao
DNA, Fungal
Fungal Proteins
Genome, Fungal
Phylogeny
Plant Diseases
Whole Genome Sequencing
URI: https://hdl.handle.net/10216/120518
Source: BMC Genomics, vol. 19(1):509
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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