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Author(s): Ribeiro T.
Lemos F.
Preto M.
Azevedo J.
Sousa M.L.
Leão P.N.
Campos A.
Linder S.
Vitorino R.
Vasconcelos V.
Urbatzka R.
Title: Cytotoxicity of portoamides in human cancer cells and analysis of the molecular mechanisms of action
Publisher: Public Library of Science
Issue Date: 2017
Abstract: Portoamides are cyclic peptides produced and released by the cyanobacterial strain Phormidium sp. presumably to interfere with other organisms in their ecosystems ("allelopathy"). Portoamides were previously demonstrated to have an antiproliferative effect on human lung carcinoma cells, but the underlying mechanism of this activity has not been described. In the present work, the effects of portoamides on proliferation were examined in eight human cancer cell lines and two non-carcinogenic cell lines, and major differences in sensitivities were observed. To generate hypotheses with regard to molecular mechanisms of action, quantitative proteomics using 2D gel electrophoresis and MALDI-TOF/TOF were performed on the colon carcinoma cell line HT-29. The expression of proteins involved in energy metabolism (mitochondrial respiratory chain and pentose phosphate pathway) was found to be affected. The hypothesis of altered energy metabolism was tested in further experiments. Exposure to portoamides resulted in reduced cellular ATP content, likely due to decreased mitochondrial energy production. Mitochondrial hyperpolarization and reduced mitochondrial reductive capacity was observed in treated cells. Furthermore, alterations in the expression of peroxiredoxins (PRDX4, PRDX6) and components of proteasome subunits (PSB4, PSA6) were observed in portoamide-treated cells, but these alterations were not associated with detectable increases in oxidative stress. We conclude that the cytotoxic activity of portoamides is associated with disturbance of energy metabolism, and alterations in mitochondrial structure and function. © 2017 Ribeiro et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Subject: adenosine triphosphate
antineoplastic agent
pentose phosphate
peroxiredoxin 4
peroxiredoxin 6
portoamide A
portoamide B
protein PSB 4
protein PSB 6
unclassified drug
adenosine triphosphate
antineoplastic activity
cancer cell line
cell proliferation
cell structure
controlled study
drug cytotoxicity
drug mechanism
drug sensitivity
energy metabolism
HT-29 cell line
human cell
matrix assisted laser desorption ionization time of flight mass spectrometry
mitochondrial respiration
oxidative stress
protein expression
respiratory chain
matrix-assisted laser desorption-ionization mass spectrometry
tumor cell line
Adenosine Triphosphate
Cell Line, Tumor
Energy Metabolism
Oxidative Stress
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Source: PLoS ONE, vol. 12(12): e0188817
Related Information: info:eu-repo/grantAgreement/FCT/5876/147268/PT
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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