Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120509
Author(s): Ramos V.M.C.
Castelo-Branco R.
Leão P.N.
Martins J.
Carvalhal-Gomes S.
da Silva F.S.
Filho J.G.M.
Vasconcelos V.M.
Title: Cyanobacterial diversity in microbial mats from the hypersaline lagoon system of Araruama, Brazil: An in-depth polyphasic study
Publisher: Frontiers Media
Issue Date: 2017
Abstract: Microbial mats are complex, micro-scale ecosystems that can be found in a wide range of environments. In the top layer of photosynthetic mats from hypersaline environments, a large diversity of cyanobacteria typically predominates. With the aim of strengthening the knowledge on the cyanobacterial diversity present in the coastal lagoon system of Araruama (state of Rio de Janeiro, Brazil), we have characterized three mat samples by means of a polyphasic approach. We have used morphological and molecular data obtained by culture-dependent and -independent methods. Moreover, we have compared different classification methodologies and discussed the outcomes, challenges, and pitfalls of these methods. Overall, we show that Araruama's lagoons harbor a high cyanobacterial diversity. Thirty-six unique morphospecies could be differentiated, which increases by more than 15% the number of morphospecies and genera already reported for the entire Araruama system. Morphology-based data were compared with the 16S rRNA gene phylogeny derived from isolate sequences and environmental sequences obtained by PCR-DGGE and pyrosequencing. Most of the 48 phylotypes could be associated with the observed morphospecies at the order level. More than one third of the sequences demonstrated to be closely affiliated (best BLAST hit results of ≥99%) with cyanobacteria from ecologically similar habitats. Some sequences had no close relatives in the public databases, including one from an isolate, being placed as "loner" sequences within different orders. This hints at hidden cyanobacterial diversity in the mats of the Araruama system, while reinforcing the relevance of using complementary approaches to study cyanobacterial diversity. © 2017 Ramos, Castelo-Branco, Leão, Martins, Carvalhal-Gomes, Sobrinho da Silva, Mendonça Filho and Vasconcelos.
Subject: RNA 16S
Amazonas (Brazil)
amplicon
Article
Chloroflexus
cyanobacterium
DNA isolation
environmental factor
gel electrophoresis
gene amplification
hypersaline lake
lagoon
lake ecosystem
microbial diversity
microbial mat
microbial morphology
microscopy
molecular cloning
molecular phylogeny
morphospecies
nonhuman
phylogeny
polymerase chain reaction
pyrosequencing
Sanger sequencing
sequence analysis
URI: https://hdl.handle.net/10216/120509
Source: Frontiers in Microbiology, vol. 8:1233
Related Information: info:eu-repo/grantAgreement/FCT/5876/147268/PT
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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