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Author(s): Ramos A.
Castro-Carvalho B.
Prata-Sena M.
Dethoup T.
Buttachon S.
Kijjoa A.
Rocha E.
Title: Crude extracts of marine-derived and soil fungi of the genus Neosartorya exhibit selective anticancer activity by inducing cell death in colon, breast and skin cancer cell lines
Publisher: Medknow Publications
Issue Date: 2016
Abstract: Background: The crude ethyl acetate extracts of marine-derived fungi Neosartorya tsunodae KUFC 9213 (E1) and N. laciniosa KUFC 7896 (E2), and soil fungus N. fischeri KUFC 6344 (E3) were evaluated for their in vitro anticancer activities on a panel of seven human cancer cell lines. Materials and Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed, after 48 h treatments with different concentrations of extracts, to determine their concentration of the extract or Dox that inhibits cell viability by 50% for each cell line. The effects of the crude extracts on DNA damage, clonogenic potential and their ability to induce cell death were also assessed. Results: E1 was found to the void of anti-proliferative effects. E2 was shown to decrease the clonogenic potential in human colorectal carcinoma cell line (HCT116), human malignant melanoma cell line (A375), human breast adenocarcinoma cell line (MCF7), and human caucasian colon adenocarcinoma Grade II cell line (HT29) cells, whereas E3 showed such effect only in HCT116 and MCF7 cells. Both extracts were found to increase DNA damage in some cell lines. E2 was found to induce cell death in HT29, HCT116, MCF7, and A375 cells while extract E3 increased cell death in MCF7 and HCT116 cell lines. Conclusion: The results reveal that E2 and E3 possess anticancer activities in human colon carcinoma, breast adenocarcinoma, and melanoma cells, validating the interest for an identification of molecular targets involved in the anticancer activity.
Subject: antineoplastic agent
fungal extract
neosartorya fischeri extract
neosartorya laciniosa extract
neosartorya tsunodae extract
unclassified drug
A375 cell line
antineoplastic activity
antiproliferative activity
breast cancer
cancer cell line
cell death
cell viability
colon cancer
DNA damage
drug potency
HCT116 cell line
HT 29 cell line
human cell
in vitro study
MCF 7 cell line
Neosartorya fischeri
Neosartorya laciniosa
Neosartorya tsunodae
skin cancer
Source: Pharmacognosy Research, vol. 8(1), p. 8-15
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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