Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120486
Author(s): Lopes-Marques M.
Kabeya N.
Qian Y.
Ruivo R.
Santos M.M.
Venkatesh B.
Tocher D.R.
Castro L.F.C.
Monroig O.
Title: Retention of fatty acyl desaturase 1 (fads1) in Elopomorpha and Cyclostomata provides novel insights into the evolution of long-chain polyunsaturated fatty acid biosynthesis in vertebrates 06 Biological Sciences 0604 Genetics 06 Biological Sciences 0601 Biochemistry and Cell Biology 06 Biological Sciences 0603 Evolutionary Biology
Publisher: BMC
Issue Date: 2018
Abstract: Background: Provision of long-chain polyunsaturated fatty acids (LC-PUFA) in vertebrates occurs through the diet or via endogenous production from C18 precursors through consecutive elongations and desaturations. It has been postulated that the abundance of LC-PUFA in the marine environment has remarkably modulated the gene complement and function of Fads in marine teleosts. In vertebrates two fatty acyl desaturases, namely Fads1 and Fads2, encode 5 and 6 desaturases, respectively. To fully clarify the evolutionary history of LC-PUFA biosynthesis in vertebrates, we investigated the gene repertoire and function of Fads from species placed at key evolutionary nodes. Results: We demonstrate that functional Fads1Δ5 and Fads26 arose from a tandem gene duplication in the ancestor of vertebrates, since they are present in the Arctic lamprey. Additionally, we show that a similar condition was retained in ray-finned fish such as the Senegal bichir and spotted gar, with the identification of fads1 genes in these lineages. Functional characterisation of the isolated desaturases reveals the first case of a Fads1 enzyme with 5 desaturase activity in the Teleostei lineage, the Elopomorpha. In contrast, in Osteoglossomorpha genomes, while no fads1 was identified, two separate fads2 duplicates with 6 and 5 desaturase activities respectively were uncovered. Conclusions: We conclude that, while the essential genetic components involved LC-PUFA biosynthesis evolved in the vertebrate ancestor, the full completion of the LC-PUFA biosynthesis pathway arose uniquely in gnathostomes. © 2018 The Author(s).
Subject: Actinopterygii
Elopomorpha
Lepisosteus oculatus
Lethenteron japonicum
Osteoglossomorpha
Teleostei
Vertebrata
acyl coenzyme A desaturase
unsaturated fatty acid
amino acid sequence
animal
biosynthesis
chemistry
fish
genetics
metabolism
molecular evolution
phylogeny
sequence homology
Amino Acid Sequence
Animals
Evolution, Molecular
Fatty Acid Desaturases
Fatty Acids, Unsaturated
Fishes
Phylogeny
Sequence Homology, Nucleic Acid
URI: https://hdl.handle.net/10216/120486
Source: BMC Evolutionary Biology, vol. 18(1):157
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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