Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120478
Author(s): Lage O.M.
Ramos M.C.
Calisto R.
Almeida E.
Vasconcelos V.
Vicente F.
Title: Current screening methodologies in drug discovery for selected human diseases
Publisher: MDPI
Issue Date: 2018
Abstract: The increase of many deadly diseases like infections by multidrug-resistant bacteria implies re-inventing the wheel on drug discovery. A better comprehension of the metabolisms and regulation of diseases, the increase in knowledge based on the study of disease-born microorganisms’ genomes, the development of more representative disease models and improvement of techniques, technologies, and computation applied to biology are advances that will foster drug discovery in upcoming years. In this paper, several aspects of current methodologies for drug discovery of antibacterial and antifungals, anti-tropical diseases, antibiofilm and antiquorum sensing, anticancer and neuroprotectors are considered. For drug discovery, two different complementary approaches can be applied: classical pharmacology, also known as phenotypic drug discovery, which is the historical basis of drug discovery, and reverse pharmacology, also designated target-based drug discovery. Screening methods based on phenotypic drug discovery have been used to discover new natural products mainly from terrestrial origin. Examples of the discovery of marine natural products are provided. A section on future trends provides a comprehensive overview on recent advances that will foster the pharmaceutical industry. © 2018 by the authors.
Subject: adenosine triphosphate
antifungal agent
antiinfective agent
antineoplastic agent
natural product
neuroprotective agent
biological product
drug
agar diffusion
anti parasitic assay
antibiofilm formation
assay
biological activity
bioluminescence
cell assay
cell proliferation assay
clonogenic cell survival assay
degenerative disease
diagnostic procedure
dilution
disk diffusion
drug development
drug screening
dye exclusion assay
enzyme assay
flask incubation assay
flow cytometry
helminth
high throughput screening
human
infection
Kinetoplastida
malaria
malignant neoplasm
marine environment
metabolism
nervous system development
neuroprotection
nonhuman
parasitosis
protease viability marker assay
quorum sensing
quorum sensing assay
Review
stained viable cell assay
thin layer chromatography
time kill test
trend study
animal
drug development
drug industry
procedures
Animals
Biological Products
Drug Discovery
Drug Industry
Humans
Pharmaceutical Preparations
URI: https://hdl.handle.net/10216/120478
Source: Marine Drugs, vol. 16(8): Y
Related Information: info:eu-repo/grantAgreement/EC/H2020/692419/EU
info:eu-repo/grantAgreement/FCT/5876/147268/PT
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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