Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120474
Author(s): Amaro H.M.
Catarina Guedes A.
Preto M.A.C.
Sousa-Pinto I.
Xavier Malcata F.
Title: Gloeothece sp. as a nutraceutical source-an improved method of extraction of carotenoids and fatty acids
Publisher: MDPI
Issue Date: 2018
Abstract: The nutraceutical potential of microalgae boomed with the exploitation of new species and sustainable extraction systems of bioactive compounds. Thus, a laboratory-made continuous pressurized solvent extraction system (CPSE) was built to optimize the extraction of antioxidant compounds, such as carotenoids and PUFA, from a scarcely studied prokaryotic microalga, Gloeothece sp. Following "green chemical principles" and using a GRAS solvent (ethanol), biomass amount, solvent flow-rate/pressure, temperature and solvent volume-including solvent recirculation-were sequentially optimized, with the carotenoids and PUFA content and antioxidant capacity being the objective functions. Gloeothece sp. bioactive compounds were best extracted at 60?C and 180 bar. Recirculation of solvent in several cycles (C) led to an 11-fold extraction increase of ß-carotene (3C) and 7.4-fold extraction of C18:2 n6 t (5C) when compared to operation in open systems. To fully validate results CPSE, this system was compared to a conventional extraction method, ultrasound assisted extraction (UAE). CPSE proved superior in extraction yield, increasing total carotenoids extraction up 3-fold and total PUFA extraction by ca. 1.5-fold, with particular extraction increase of 18:3 n3 by 9.6-fold. Thus, CPSE proved to be an efficient and greener extraction method to obtain bioactive extract from Gloeothece sp. for nutraceutical purposes-with low levels of resources spent, while lowering costs of production and environmental impacts. © 2018 by the authors.
Subject: alcohol
beta carotene
carotenoid
nutraceutical
polyunsaturated fatty acid
antioxidant
biological product
carotenoid
fatty acid
ABTS radical scavenging assay
antioxidant assay
Article
biomass production
chemical composition
chemical parameters
continuous pressurized solvent extraction
controlled study
DPPH radical scavenging assay
environmental impact
flow rate
Gloeothece
green chemistry
intermethod comparison
microalga
nonhuman
process optimization
solvent effect
solvent extraction
solvent volume
temperature dependence
ultrasound assisted extraction
biomass
chemistry
comparative study
cyanobacterium
dietary supplement
economics
isolation and purification
liquid liquid extraction
microalga
procedures
temperature
ultrasound
validation study
Antioxidants
Biological Products
Biomass
Carotenoids
Cyanobacteria
Dietary Supplements
Ethanol
Fatty Acids
Green Chemistry Technology
Liquid-Liquid Extraction
Microalgae
Temperature
Ultrasonic Waves
URI: https://hdl.handle.net/10216/120474
Source: Marine Drugs, vol. 16(9):327
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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