Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120470
Author(s): Humisto A.
Jokela J.
Liu L.
Wahlsten M.
Wang H.
Permi P.
Machado J.P.
Antunes A.
Fewer D.P.
Sivonen K.
Title: The swinholide biosynthesis gene cluster from a terrestrial cyanobacterium, Nostoc sp. strain UHCC 0450
Publisher: American Society for Microbiology
Issue Date: 2018
Abstract: Swinholides are 42-carbon ring polyketides with a 2-fold axis of symmetry. They are potent cytotoxins that disrupt the actin cytoskeleton. Swinholides were discovered from the marine sponge Theonella sp. and were long suspected to be produced by symbiotic bacteria. Misakinolide, a structural variant of swinholide, was recently demonstrated to be the product of a symbiotic heterotrophic proteobacterium. Here, we report the production of swinholide A by an axenic strain of the terrestrial cyanobacterium Nostoc sp. strain UHCC 0450. We located the 85-kb trans-AT polyketide synthase (PKS) swinholide biosynthesis gene cluster from a draft genome of Nostoc sp. UHCC 0450. The swinholide and misakinolide biosynthesis gene clusters share an almost identical order of catalytic domains, with 85% nucleotide sequence identity, and they group together in phylogenetic analysis. Our results resolve speculation around the true producer of swinholides and demonstrate that bacteria belonging to two distantly related phyla both produce structural variants of the same natural product. In addition, we described a biosynthesis cluster from Anabaena sp. strain UHCC 0451 for the synthesis of the cytotoxic and antifungal scytophycin. All of these biosynthesis gene clusters were closely related to each other and created a group of cytotoxic macrolide compounds produced by trans-AT PKSs of cyanobacteria and proteobacteria. © 2018 American Society for Microbiology.
Subject: Bacteria
Biochemistry
Biosynthesis
Gene transfer
Genes
Ketones
Proteins
Anabaena
Candidatus Entotheonella
Cyanobacteria
Horizontal gene transfer
Nostoc
Polyketides
Scytophycin
Swinholide
Trans-AT PKS
Scandium compounds
bacterium
bovine spongiform encephalopathy
catalysis
catalyst
cyanobacterium
enzyme
enzyme activity
gene
gene expression
gene transfer
marine environment
phylogenetics
skeleton
terrestrial environment
toxin
Anabaena
Anabaena sp.
Candidatus Entotheonella
Cyanobacteria
Nostoc
Nostoc sp.
Proteobacteria
Theonella sp.
URI: https://hdl.handle.net/10216/120470
Source: Applied and Environmental Microbiology, vol. 84(3): e02321-17
Related Information: info:eu-repo/grantAgreement/FCT/5876/147268/PT
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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