Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/120470
Author(s): | Humisto A. Jokela J. Liu L. Wahlsten M. Wang H. Permi P. Machado J.P. Antunes A. Fewer D.P. Sivonen K. |
Title: | The swinholide biosynthesis gene cluster from a terrestrial cyanobacterium, Nostoc sp. strain UHCC 0450 |
Publisher: | American Society for Microbiology |
Issue Date: | 2018 |
Abstract: | Swinholides are 42-carbon ring polyketides with a 2-fold axis of symmetry. They are potent cytotoxins that disrupt the actin cytoskeleton. Swinholides were discovered from the marine sponge Theonella sp. and were long suspected to be produced by symbiotic bacteria. Misakinolide, a structural variant of swinholide, was recently demonstrated to be the product of a symbiotic heterotrophic proteobacterium. Here, we report the production of swinholide A by an axenic strain of the terrestrial cyanobacterium Nostoc sp. strain UHCC 0450. We located the 85-kb trans-AT polyketide synthase (PKS) swinholide biosynthesis gene cluster from a draft genome of Nostoc sp. UHCC 0450. The swinholide and misakinolide biosynthesis gene clusters share an almost identical order of catalytic domains, with 85% nucleotide sequence identity, and they group together in phylogenetic analysis. Our results resolve speculation around the true producer of swinholides and demonstrate that bacteria belonging to two distantly related phyla both produce structural variants of the same natural product. In addition, we described a biosynthesis cluster from Anabaena sp. strain UHCC 0451 for the synthesis of the cytotoxic and antifungal scytophycin. All of these biosynthesis gene clusters were closely related to each other and created a group of cytotoxic macrolide compounds produced by trans-AT PKSs of cyanobacteria and proteobacteria. © 2018 American Society for Microbiology. |
Subject: | Bacteria Biochemistry Biosynthesis Gene transfer Genes Ketones Proteins Anabaena Candidatus Entotheonella Cyanobacteria Horizontal gene transfer Nostoc Polyketides Scytophycin Swinholide Trans-AT PKS Scandium compounds bacterium bovine spongiform encephalopathy catalysis catalyst cyanobacterium enzyme enzyme activity gene gene expression gene transfer marine environment phylogenetics skeleton terrestrial environment toxin Anabaena Anabaena sp. Candidatus Entotheonella Cyanobacteria Nostoc Nostoc sp. Proteobacteria Theonella sp. |
URI: | https://hdl.handle.net/10216/120470 |
Source: | Applied and Environmental Microbiology, vol. 84(3): e02321-17 |
Related Information: | info:eu-repo/grantAgreement/FCT/5876/147268/PT |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | openAccess |
Appears in Collections: | CIIMAR - Artigo em Revista Científica Internacional |
Files in This Item:
File | Description | Size | Format | |
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Humisto A_2018.pdf | 3.05 MB | Adobe PDF | View/Open |
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