Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/120461
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Freitas S. | |
dc.creator | Martins R. | |
dc.creator | Costa M. | |
dc.creator | Leão P.N. | |
dc.creator | Vitorino R. | |
dc.creator | Vasconcelos V. | |
dc.creator | Urbatzka R. | |
dc.date.accessioned | 2019-05-31T16:16:00Z | - |
dc.date.available | 2019-05-31T16:16:00Z | - |
dc.date.issued | 2016 | |
dc.identifier.issn | 16603397 | |
dc.identifier.uri | https://hdl.handle.net/10216/120461 | - |
dc.description.abstract | Background: Hierridin B was isolated from a marine cyanobacterium Cyanobium sp. strain and induced cytotoxicity selectively in HT-29 adenocarcinoma cells. The underlying molecular mechanism was not yet elucidated. Methods: HT-29 cells were exposed to the IC50concentration of hierridin B (100.2 μM) for 48 h. Non-targeted proteomics was performed using 2D gel electrophoresis and MALDI-TOF/TOF mass spectrometry. The mRNA expression of apoptotic and cell cycle genes were analyzed by real-time PCR. Automated quantification of 160 cytoplasm and mitochondrial parameter was done by fluorescence microscopy using CellProfiler software. Results: Proteomics identified 21 significant different proteins, which belonged to protein folding/synthesis and cell structure amongst others. Increase of VDAC1 protein responsible for formation of mitochondrial channels was confirmed by mRNA expression. A 10-fold decrease of cytoskeleton proteins (STMN1, TBCA) provided a link to alterations of the cell cycle. CCNB1 and CCNE mRNA were decreased two-fold, and P21CIP increased 10-fold, indicative of cell cycle arrest. Morphological analysis of mitochondrial parameter confirmed a reduced mitochondrial activity. Conclusion: Hierridin B is a potential anticancer compound that targets mitochondrial activity and function. | |
dc.description.sponsorship | This research was partially supported by FCT—Foundation for Science and Technology under the project UID/Multi/04423/2013 and by the Structured Program of R & D & I INNOVMAR—Innovation and Sustainability in the Management and Exploitation of Marine Resources (reference NORTE-01-0145-FEDER-000035, Research Line NOVELMAR), funded by the Northern Regional Operational Program (NORTE2020) through the European Regional Development Fund (ERDF). Ralph Urbatzka was supported by the FCT scholarship SFRH/BPD/112287/2015, and Pedro N. Leão by FCT grant IF/01358/2014. | |
dc.language.iso | eng | |
dc.publisher | MDPI | |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147268/PT | |
dc.relation.ispartof | Marine Drugs, vol. 14(9):158 | |
dc.rights | openAccess | |
dc.title | Hierridin B Isolated from a Marine Cyanobacterium Alters VDAC1, Mitochondrial Activity, and Cell Cycle Genes on HT-29 Colon Adenocarcinoma Cells | |
dc.type | Artigo em Revista Científica Internacional | |
dc.contributor.uporto | CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental | |
dc.identifier.doi | 10.3390/md14090158 | |
dc.relation.publisherversion | http://dx.doi.org/10.3390/md14090158 | |
Appears in Collections: | CIIMAR - Artigo em Revista Científica Internacional |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Freitas S_2_2016.pdf | 3.53 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.