Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120461
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dc.creatorFreitas S.
dc.creatorMartins R.
dc.creatorCosta M.
dc.creatorLeão P.N.
dc.creatorVitorino R.
dc.creatorVasconcelos V.
dc.creatorUrbatzka R.
dc.date.accessioned2019-05-31T16:16:00Z-
dc.date.available2019-05-31T16:16:00Z-
dc.date.issued2016
dc.identifier.issn16603397
dc.identifier.urihttps://hdl.handle.net/10216/120461-
dc.description.abstractBackground: Hierridin B was isolated from a marine cyanobacterium Cyanobium sp. strain and induced cytotoxicity selectively in HT-29 adenocarcinoma cells. The underlying molecular mechanism was not yet elucidated. Methods: HT-29 cells were exposed to the IC50concentration of hierridin B (100.2 μM) for 48 h. Non-targeted proteomics was performed using 2D gel electrophoresis and MALDI-TOF/TOF mass spectrometry. The mRNA expression of apoptotic and cell cycle genes were analyzed by real-time PCR. Automated quantification of 160 cytoplasm and mitochondrial parameter was done by fluorescence microscopy using CellProfiler software. Results: Proteomics identified 21 significant different proteins, which belonged to protein folding/synthesis and cell structure amongst others. Increase of VDAC1 protein responsible for formation of mitochondrial channels was confirmed by mRNA expression. A 10-fold decrease of cytoskeleton proteins (STMN1, TBCA) provided a link to alterations of the cell cycle. CCNB1 and CCNE mRNA were decreased two-fold, and P21CIP increased 10-fold, indicative of cell cycle arrest. Morphological analysis of mitochondrial parameter confirmed a reduced mitochondrial activity. Conclusion: Hierridin B is a potential anticancer compound that targets mitochondrial activity and function.
dc.description.sponsorshipThis research was partially supported by FCT—Foundation for Science and Technology under the project UID/Multi/04423/2013 and by the Structured Program of R & D & I INNOVMAR—Innovation and Sustainability in the Management and Exploitation of Marine Resources (reference NORTE-01-0145-FEDER-000035, Research Line NOVELMAR), funded by the Northern Regional Operational Program (NORTE2020) through the European Regional Development Fund (ERDF). Ralph Urbatzka was supported by the FCT scholarship SFRH/BPD/112287/2015, and Pedro N. Leão by FCT grant IF/01358/2014.
dc.language.isoeng
dc.publisherMDPI
dc.relationinfo:eu-repo/grantAgreement/FCT/5876/147268/PT
dc.relation.ispartofMarine Drugs, vol. 14(9):158
dc.rightsopenAccess
dc.titleHierridin B Isolated from a Marine Cyanobacterium Alters VDAC1, Mitochondrial Activity, and Cell Cycle Genes on HT-29 Colon Adenocarcinoma Cells
dc.typeArtigo em Revista Científica Internacional
dc.contributor.uportoCIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental
dc.identifier.doi10.3390/md14090158
dc.relation.publisherversionhttp://dx.doi.org/10.3390/md14090158
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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