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Author(s): Valério E.
Campos A.
Osório H.
Vasconcelos V.
Title: Proteomic and Real-Time PCR analyses of Saccharomyces cerevisiae VL3 exposed to microcystin-LR reveals a set of protein alterations transversal to several eukaryotic models
Publisher: Elsevier
Issue Date: 2016
Abstract: Some of the most common toxins present in freshwater, in particular microcystins (MCs), are produced by cyanobacteria. These toxins have a negative impact on human health, being associated with episodes of acute hepatotoxicity and being considered potentially carcinogenic to humans. To date the exact mechanisms of MC-induced toxicity and tumor promotion were not completely elucidated. To get new insights underlying microcystin-LR (MCLR) molecular mechanisms of toxicity we have performed the proteomic profiling using two-dimensional electrophoresis and MALDI-TOF/TOF of Saccharomyces cerevisiae cells exposed for 4 h-1 nM and 1 μM of MCLR, and compared them to the control (cells not exposed to MCLR). We identified 14 differentially expressed proteins. The identified proteins are involved in metabolism, genotoxicity, cytotoxicity and stress response. Furthermore, we evaluated the relative expression of yeast's PP1 and PP2A genes and also of genes from the Base Excision Repair (BER) DNA-repair system, and observed that three out of the five genes analyzed displayed dose-dependent responses. Overall, the different proteins and genes affected are related to oxidative stress and apoptosis, thus reinforcing that it is probably the main mechanism of MCLR toxicity transversal to several organisms, especially at lower doses. Notwithstanding these MCLR responsive proteins could be object of further studies to evaluate their suitability as biomarkers of exposure to the toxin. © 2016 Elsevier. All rights reserved.
Subject: fungal protein
microcystin LR
ALG9 gene
CDC55 gene
concentration (parameters)
controlled study
DNA repair
eukaryotic cell
excision repair
fungal gene
gene expression
GLC7 gene
matrix assisted laser desorption ionization time of flight mass spectrometry
molecular dynamics
NTG1 gene
NTG2 gene
oxidative stress
PP1 gene
PP2A gene
priority journal
protein expression
protein modification
real time polymerase chain reaction
regulatory mechanism
Saccharomyces cerevisiae
TAF10 gene
two dimensional electrophoresis
Source: Toxicon, vol. 112, p. 22-28
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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