Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/120426
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Ribeiro H. | |
dc.creator | de Sousa T. | |
dc.creator | Santos J.P. | |
dc.creator | Sousa A.G.G. | |
dc.creator | Teixeira C. | |
dc.creator | Monteiro M.R. | |
dc.creator | Salgado P. | |
dc.creator | Mucha A.P. | |
dc.creator | Almeida C.M.R. | |
dc.creator | Torgo L. | |
dc.creator | Magalhães C. | |
dc.date.accessioned | 2019-05-31T16:15:39Z | - |
dc.date.available | 2019-05-31T16:15:39Z | - |
dc.date.issued | 2018 | |
dc.identifier.issn | 456535 | |
dc.identifier.uri | https://hdl.handle.net/10216/120426 | - |
dc.description.abstract | This study investigates the potential of an indigenous estuarine microbial consortium to degrade two polycyclic aromatic hydrocarbons (PAHs), naphthalene and fluoranthene, under nitrate-reducing conditions. Two physicochemically diverse sediment samples from the Lima Estuary (Portugal) were spiked individually with 25 mg L−1 of each PAH in laboratory designed microcosms. Sediments without PAHs and autoclaved sediments spiked with PAHs were run in parallel. Destructive sampling at the beginning and after 3, 6, 12, 30 and 63 weeks incubation was performed. Naphthalene and fluoranthene levels decreased over time with distinct degradation dynamics varying with sediment type. Next-generation sequencing (NGS) of 16 S rRNA gene amplicons revealed that the sediment type and incubation time were the main drivers influencing the microbial community structure rather than the impact of PAH amendments. Predicted microbial functional analyses revealed clear shifts and interrelationships between genes involved in anaerobic and aerobic degradation of PAHs and in the dissimilatory nitrate-reducing pathways (denitrification and dissimilatory nitrate reduction to ammonium - DNRA). These findings reinforced by clear biogeochemical denitrification signals (NO3 − consumption, and NH4 + increased during the incubation period), suggest that naphthalene and fluoranthene degradation may be coupled with denitrification and DNRA metabolism. The results of this study contribute to the understanding of the dissimilatory nitrate-reducing pathways and help uncover their involvement in degradation of PAHs, which will be crucial for directing remediation strategies of PAH-contaminated anoxic sediments. © 2018 Elsevier | |
dc.description.sponsorship | The authors acknowledge the reviewers for their valuable comments and suggestions, which were helpful in improving the manuscript. This research was partially supported by the Structured Program of R&D&I INNOVMAR - Innovation and Sustainability in the Management and Exploitation of Marine Resources . The post-doctoral scholarship granted by Erasmus Mundus Interweave to T. de Sousa is greatly acknowledged. C. Magalhães acknowledges Investigator FCT program supported by FCT, funded by the European Social Fund through the Operational Program of Human Capital (POCH) . C. Teixeira acknowledges FCT for a postdoctoral grant (ref. SFRH/BPD/110730/2015 ) cofunded by MCTES and the European Social Fund through POCH/QEC . Appendix A | |
dc.language.iso | eng | |
dc.publisher | Elsevier | |
dc.relation.ispartof | Chemosphere, vol. 199, p. 54-67 | |
dc.rights | restrictedAccess | |
dc.subject | Anoxic sediments | |
dc.subject | Aromatization | |
dc.subject | Denitrification | |
dc.subject | Genes | |
dc.subject | Hydrocarbons | |
dc.subject | Naphthalene | |
dc.subject | Nitrates | |
dc.subject | RNA | |
dc.subject | Degradation dynamics | |
dc.subject | Dissimilatory nitrate reduction | |
dc.subject | Fluoranthene | |
dc.subject | Microbial community structures | |
dc.subject | Next-generation sequencing | |
dc.subject | Nitrate-reducing conditions | |
dc.subject | Polycyclic aromatic hydrocarbons (PAHS) | |
dc.subject | Remediation strategies | |
dc.subject | Polycyclic aromatic hydrocarbons | |
dc.subject | ammonia | |
dc.subject | fluoranthene | |
dc.subject | methane | |
dc.subject | naphthalene | |
dc.subject | nitrate | |
dc.subject | nitrogen | |
dc.subject | organic matter | |
dc.subject | polycyclic aromatic hydrocarbon | |
dc.subject | ribosome RNA | |
dc.subject | ammonium derivative | |
dc.subject | nitric acid derivative | |
dc.subject | polycyclic aromatic hydrocarbon | |
dc.subject | ammonium | |
dc.subject | biodegradation | |
dc.subject | community structure | |
dc.subject | degradation | |
dc.subject | denitrification | |
dc.subject | estuarine dynamics | |
dc.subject | fluoranthene | |
dc.subject | metabolism | |
dc.subject | microbial activity | |
dc.subject | nitrate | |
dc.subject | PAH | |
dc.subject | reduction | |
dc.subject | amplicon | |
dc.subject | Article | |
dc.subject | biodegradation | |
dc.subject | community structure | |
dc.subject | controlled study | |
dc.subject | denitrification | |
dc.subject | incubation time | |
dc.subject | metabolism | |
dc.subject | microbial community | |
dc.subject | microbial consortium | |
dc.subject | microcosm | |
dc.subject | next generation sequencing | |
dc.subject | reduction (chemistry) | |
dc.subject | sediment | |
dc.subject | bioremediation | |
dc.subject | chemistry | |
dc.subject | denitrification | |
dc.subject | estuary | |
dc.subject | Portugal | |
dc.subject | Limia Estuary | |
dc.subject | Portugal | |
dc.subject | Viana do Castelo [Portugal] | |
dc.subject | Ammonium Compounds | |
dc.subject | Biodegradation, Environmental | |
dc.subject | Denitrification | |
dc.subject | Estuaries | |
dc.subject | Geologic Sediments | |
dc.subject | Microbial Consortia | |
dc.subject | Nitrates | |
dc.subject | Polycyclic Aromatic Hydrocarbons | |
dc.subject | Portugal | |
dc.title | Potential of dissimilatory nitrate reduction pathways in polycyclic aromatic hydrocarbon degradation | |
dc.type | Artigo em Revista Científica Internacional | |
dc.contributor.uporto | CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental | |
dc.identifier.doi | 10.1016/j.chemosphere.2018.01.171 | |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.chemosphere.2018.01.171 | |
Appears in Collections: | CIIMAR - Artigo em Revista Científica Internacional |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Ribeiro H_2018.pdf Restricted Access | 2.62 MB | Adobe PDF | Request a copy |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.