Please use this identifier to cite or link to this item:
Author(s): Peixoto M.J.
Domingues A.
Batista S.
Gonçalves J.F.M.
Gomes A.M.
Cunha S.
Valente L.M.P.
Costas B.
Ozório R.O.A.
Title: Physiopathological responses of sole (Solea senegalensis) subjected to bacterial infection and handling stress after probiotic treatment with autochthonous bacteria
Publisher: Elsevier
Issue Date: 2018
Abstract: This study aimed to evaluate the protective effects of four autochthonous bacteria isolated from juvenile sole (Solea senegalensis) intestine as dietary probiotic supplement against bacterial pathogen infection and handling/transport stressors. Growth performance and immune responses were evaluated after 85 days of feeding trial. Sole (IBW = 16.07 ± 0.11 g) were fed six experimental diets, a control diet (CTRL, without the dietary probiotic supplementation), and five diets supplemented with probiotic bacteria: PB1 (Shewanella hafniensis), PB2 (Enterococcus raffinosus), PB3 (Shewanella hafniensis + Arthrobacter soli), PB4 (Pseudomonas protegens + Arthrobacter soli) and PB5 (Shewanella hafniensis + Arthrobacter soli + Enterococcus raffinosus). All bacteria were selected based on their in vitro antimicrobial activity. After the growth trial, fish were submitted to a stress factor (transport) and then each dietary group was divided in two additional groups: non-infected (placebo) and infected with Photobacterium damselae subsp. piscicida. Immune and antioxidant responses were evaluated at day 10 post-infection. In infection trial A, fish were infected on the same day of transport, whereas in trial B fish were infected after a 7-day recovery from the transport stress. At the end of the feeding trial, fish fed with PB2 and PB4 showed lower final body weight when compared with the other dietary groups. Respiratory burst activity and nitric oxide production were not affected by probiotic supplementation. Fish fed with PB5 presented lower peroxidase activity compared to CTRL. Lysozyme and alternative complement pathway activity (ACH50) showed no significant differences between treatments. The innate immune responses were significantly affected after handling stress and bacterial infection. In trial A, the ACH50 levels of infected fish were significantly lower than the placebo groups. On the other hand, in trial B fish infected with Pdp demonstrated higher ACH50 levels when compared to placebos. Peroxidase levels were strongly modulated by bacterial infection and handling stress. In trials A and B, infection had a clear downgrade effect in peroxidase levels. Lipid peroxidation, catalase, glutathione S-transferase and glutathione reductase were altered by both bacterial infection and transport. Overall, dietary probiotic supplementation did not influence growth performance of sole. The immune and oxidative defenses of sole responded differently to infection depending on the probiotic and the synergy between pathogen infection and transport. © 2018
Subject: catalase
glutathione reductase
glutathione transferase
nitric oxide
probiotic agent
probiotic agent
animal experiment
animal model
animal tissue
Arthrobacter soli
bacterial infection
bacterium isolation
body weight
complement alternative pathway
controlled study
diet supplementation
Enterococcus raffinosus
in vitro study
innate immunity
lipid peroxidation
priority journal
Pseudomonas protegens
Shewanella hafniensis
Solea senegalensis
transport stress
fish disease
Gram negative infection
growth, development and aging
physiological stress
veterinary medicine
Fish Diseases
Gram-Negative Bacterial Infections
Stress, Physiological
Source: Fish and Shellfish Immunology, vol. 83, p. 348-358
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

Files in This Item:
File Description SizeFormat 
Peixoto MJ_2018.pdf
  Restricted Access
948.77 kBAdobe PDF    Request a copy

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.