Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120397
Author(s): Domínguez-Pérez D.
Campos A.
Rodríguez A.A.
Turkina M.V.
Ribeiro T.
Osorio H.
Vasconcelos V.
Antunes A.
Title: Proteomic analyses of the unexplored sea anemone Bunodactis verrucosa
Publisher: MDPI
Issue Date: 2018
Abstract: Cnidarian toxic products, particularly peptide toxins, constitute a promising target for biomedicine research. Indeed, cnidarians are considered as the largest phylum of generally toxic animals. However, research on peptides and toxins of sea anemones is still limited. Moreover, most of the toxins from sea anemones have been discovered by classical purification approaches. Recently, high-throughput methodologies have been used for this purpose but in other Phyla. Hence, the present work was focused on the proteomic analyses of whole-body extract from the unexplored sea anemone Bunodactis verrucosa. The proteomic analyses applied were based on two methods: two-dimensional gel electrophoresis combined with MALDI-TOF/TOF and shotgun proteomic approach. In total, 413 proteins were identified, but only eight proteins were identified from gel-based analyses. Such proteins are mainly involved in basal metabolism and biosynthesis of antibiotics as the most relevant pathways. In addition, some putative toxins including metalloproteinases and neurotoxins were also identified. These findings reinforce the significance of the production of antimicrobial compounds and toxins by sea anemones, which play a significant role in defense and feeding. In general, the present study provides the first proteome map of the sea anemone B. verrucosa stablishing a reference for future studies in the discovery of new compounds. © 2018 by the authors.
Subject: antibiotic agent
antiinfective agent
marine toxin
metalloproteinase
neurotoxin
protein derivative
proteome
peptide
tissue extract
Article
biosynthesis
Bunodactis verrucosa
controlled study
drug synthesis
feeding
matrix assisted laser desorption ionization time of flight mass spectrometry
nonhuman
protein determination
protein function
proteomics
sea anemone
toxin analysis
two dimensional gel electrophoresis
animal
biology
chemistry
gene ontology
genetics
matrix-assisted laser desorption-ionization mass spectrometry
microbial sensitivity test
sea anemone
Animals
Computational Biology
Gene Ontology
Metalloproteases
Microbial Sensitivity Tests
Neurotoxins
Peptides
Proteomics
Sea Anemones
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tissue Extracts
URI: https://hdl.handle.net/10216/120397
Source: Marine Drugs, vol. 16(2):42
Related Information: info:eu-repo/grantAgreement/FCT/5876/147268/PT
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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