Please use this identifier to cite or link to this item:
Author(s): Marlétaz F.
Firbas P.N.
Maeso I.
Tena J.J.
Bogdanovic O.
Perry M.
Wyatt C.D.R.
de la Calle-Mustienes E.
Bertrand S.
Burguera D.
Acemel R.D.
van Heeringen S.J.
Naranjo S.
Herrera-Ubeda C.
Skvortsova K.
Jimenez-Gancedo S.
Aldea D.
Marquez Y.
Buono L.
Kozmikova I.
Permanyer J.
Louis A.
Albuixech-Crespo B.
Le Petillon Y.
Leon A.
Subirana L.
Balwierz P.J.
Duckett P.E.
Farahani E.
Aury J.-M.
Mangenot S.
Wincker P.
Albalat R.
Benito-Gutiérrez È.
Cañestro C.
Castro F.
D’Aniello S.
Ferrier D.E.K.
Huang S.
Laudet V.
Marais G.A.B.
Pontarotti P.
Schubert M.
Seitz H.
Somorjai I.
Takahashi T.
Mirabeau O.
Xu A.
Yu J.-K.
Carninci P.
Martinez-Morales J.R.
Crollius H.R.
Kozmik Z.
Weirauch M.T.
Garcia-Fernàndez J.
Lister R.
Lenhard B.
Holland P.W.H.
Escriva H.
Gómez-Skarmeta J.L.
Irimia M.
Title: Amphioxus functional genomics and the origins of vertebrate gene regulation
Publisher: Nature Publishing Group
Issue Date: 2018
Abstract: Vertebrates have greatly elaborated the basic chordate body plan and evolved highly distinctive genomes that have been sculpted by two whole-genome duplications. Here we sequence the genome of the Mediterranean amphioxus (Branchiostoma lanceolatum) and characterize DNA methylation, chromatin accessibility, histone modifications and transcriptomes across multiple developmental stages and adult tissues to investigate the evolution of the regulation of the chordate genome. Comparisons with vertebrates identify an intermediate stage in the evolution of differentially methylated enhancers, and a high conservation of gene expression and its cis-regulatory logic between amphioxus and vertebrates that occurs maximally at an earlier mid-embryonic phylotypic period. We analyse regulatory evolution after whole-genome duplications, and find that—in vertebrates—over 80% of broadly expressed gene families with multiple paralogues derived from whole-genome duplications have members that restricted their ancestral expression, and underwent specialization rather than subfunctionalization. Counter-intuitively, paralogues that restricted their expression increased the complexity of their regulatory landscapes. These data pave the way for a better understanding of the regulatory principles that underlie key vertebrate innovations. © 2018, Springer Nature Limited.
Subject: transcriptome
comparative study
developmental stage
gene expression
genetic analysis
animal cell
animal tissue
controlled study
developmental stage
DNA methylation
embryo development
functional genomics
gene control
gene duplication
gene expression
gene sequence
histone modification
molecular genetics
multigene family
priority journal
zebra fish
Branchiostoma lanceolatum
Source: Nature, vol. 564(7734), p. 64-70
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

Files in This Item:
File Description SizeFormat 
Marletaz F_2018.pdf
  Restricted Access
29.01 MBAdobe PDF    Request a copy

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.