Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/120379
Author(s): | Maia A.S. Castro P.M.L. Tiritan M.E. |
Title: | Integrated liquid chromatography method in enantioselective studies: Biodegradation of ofloxacin by an activated sludge consortium |
Publisher: | Elsevier |
Issue Date: | 2016 |
Abstract: | Ofloxacin is a chiral fluoroquinolone commercialized as racemate and as its enantiomerically pure form levofloxacin. This work presents an integrated liquid chromatography (LC) method with fluorescence detection (FD) and exact mass spectrometry (EMS) developed to assess the enantiomeric biodegradation of ofloxacin and levofloxacin in laboratory-scale microcosms. The optimized enantioseparation conditions were achieved using a macrocyclic antibiotic ristocetin A-bonded CSP (150 × 2.1 mm i.d.; particle size 5 μm) under reversed-phase elution mode. The method was validated using a mineral salts medium as matrix and presented selectivity and linearity over a concentration range from 5 μg L−1 (quantification limit) to 350 μg L−1 for each enantiomer. The method was successfully applied to evaluate biodegradation of ofloxacin enantiomers at 250 μg L−1 by an activated sludge inoculum. Ofloxacin (racemic mixture) and (S)-enantiomer (levofloxacin) were degraded up to 58 and 52%, respectively. An additional degradable carbon source, acetate, enhanced biodegradation up to 23%. (S)-enantiomer presented the highest extent of degradation (66.8%) when ofloxacin was supplied along with acetate. Results indicated slightly higher biodegradation extents for the (S)-enantiomer when supplementation was done with ofloxacin. Degradation occurred faster in the first 3 days and proceeded slowly until the end of the assays. The chromatographic results from LC-FD suggested the formation of the (R)-enantiomer during levofloxacin biodegradation which was confirmed by LC–MS with a LTQ Orbitrap XL. © 2016 Elsevier |
Subject: | Activated sludge process Antibiotics Carbon Chromatography Drug products Enantiomers Enantioselectivity Finite difference method Liquid chromatography Mass spectrometry Particle size Activated sludge Enhanced biodegradation Fluorescence detection Levofloxacin Macrocyclic antibiotics Mineral salts mediums Ofloxacin Stationary phasis Biodegradation acetic acid levofloxacin ofloxacin ristocetin A antiinfective agent levofloxacin sewage water pollutant acidification activated sludge aqueous solution Article biodegradation concentration (parameters) enantioselectivity exact mass spectrometry flow rate fluorescence analysis ionization limit of quantitation mass spectrometry particle size pH priority journal process optimization waste water management analysis bioremediation isolation and purification limit of detection liquid chromatography microbiology procedures sewage stereoisomerism tandem mass spectrometry validation study water pollutant Anti-Infective Agents Biodegradation, Environmental Chromatography, Liquid Levofloxacin Limit of Detection Sewage Stereoisomerism Tandem Mass Spectrometry Water Pollutants, Chemical |
URI: | https://hdl.handle.net/10216/120379 |
Source: | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 1029-1030, p. 174-183 |
Related Information: | info:eu-repo/grantAgreement/FCT/5876/147268/PT |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | restrictedAccess |
Appears in Collections: | CIIMAR - Artigo em Revista Científica Internacional |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Maia AS_2016.pdf Restricted Access | 1.05 MB | Adobe PDF | Request a copy |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.