Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120376
Author(s): Madureira T.V.
Malhão F.
Simões T.
Pinheiro I.
Lopes C.
Gonçalves J.F.
Urbatzka R.
Castro L.F.C.
Lemos M.F.L.
Rocha E.
Title: Sex-steroids and hypolipidemic chemicals impacts on brown trout lipid and peroxisome signaling — Molecular, biochemical and morphological insights
Publisher: Elsevier
Issue Date: 2018
Abstract: Lipid metabolism involves complex pathways, which are regulated in a similar way across vertebrates. Hormonal and hypolipidemic deregulations cause lipid imbalance from fish to humans, but the underlying mechanisms are far from understood. This study explores the potential of using juvenile brown trout to evaluate the in vivo interferences caused by estrogenic (17α-ethinylestradiol – EE2), androgenic (testosterone – T), and hypolipidemic (clofibrate – CLF) compounds in lipidic and/or peroxisomal pathways. Studied endpoints were from blood/plasma biochemistry, plasma fatty acid profile, ultrastructure of hepatocytes and abundance of their peroxisomes to mRNA expression in the liver. Both T and CLF caused minimal effects when compared to EE2. Estrogenized fish had significantly higher hepatosomatic indexes, increased triglycerides and very-low density lipoproteins (VLDL) in plasma, compared with solvent control. Morphologically, EE2 fish showed increased lipid droplets in hepatocytes, and EE2 and T reduced volume density of peroxisomes in relation to the hepatic parenchyma. Polyunsaturated fatty acids (PUFA) in plasma, namely n−3 PUFA, increased with EE2. EE2 animals had increased mRNA levels of vitellogenin A (VtgA), estrogen receptor alpha (ERα), peroxisome proliferator-activated receptor alpha (PPARα), PPARαBa and acyl-CoA long chain synthetase 1 (Acsl1), while ERβ-1, acyl-CoA oxidase 1–3I (Acox1–3I), Acox3, PPARγ, catalase (Cat), urate oxidase (Uox), fatty acid binding protein 1 (Fabp1) and apolipoprotein AI (ApoAI) were down-regulated. In summary, in vivo EE2 exposure altered lipid metabolism and peroxisome dynamics in brown trout, namely by changing the mRNA levels of several genes. Our model can be used to study possible organism-level impacts, viz. in gonadogenesis. © 2018 Elsevier
Subject: acyl coenzyme A long chain synthetase 1
acyl coenzyme A oxidase 1 31
acyl coenzyme A oxidase 3
apolipoprotein A1
catalase
clofibrate
estrogen receptor alpha
estrogen receptor beta 1
ethinylestradiol
fatty acid binding protein 1
peroxisome proliferator activated receptor alpha
peroxisome proliferator activated receptor alpha Ba
peroxisome proliferator activated receptor gamma
polyunsaturated fatty acid
protein
testosterone
triacylglycerol
unclassified drug
urate oxidase
very low density lipoprotein
vitellogenin A
androgen
antilipemic agent
clofibrate
estrogen
ethinylestradiol
fat droplet
fish protein
lipid
testosterone
animal cell
animal experiment
animal tissue
Article
cell density
controlled study
down regulation
gene expression
in vivo study
lipid metabolism
lipoprotein blood level
liver cell
liver parenchyma
nonhuman
peroxisome
priority journal
Salmo trutta
signal transduction
triacylglycerol blood level
animal
aquaculture
blood
comparative study
drug effect
fluorescence microscopy
gene expression regulation
genetics
growth, development and aging
lipid metabolism
liver
metabolism
peroxisome
Portugal
randomization
salmonine
signal transduction
toxicity testing
transmission electron microscopy
ultrastructure
Androgens
Animals
Aquaculture
Clofibrate
Estrogens
Ethinyl Estradiol
Fish Proteins
Gene Expression Regulation, Developmental
Hypolipidemic Agents
Lipid Droplets
Lipid Metabolism
Lipids
Liver
Microscopy, Electron, Transmission
Microscopy, Fluorescence
Peroxisomes
Portugal
Random Allocation
Signal Transduction
Testosterone
Toxicity Tests, Subacute
Trout
URI: https://hdl.handle.net/10216/120376
Source: Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, vol. 212, p. 1-17
Related Information: info:eu-repo/grantAgreement/FCT/5876/147268/PT
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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