Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120373
Author(s): Madureira T.V.
Pinheiro I.
de Paula Freire R.
Rocha E.
Castro L.F.
Urbatzka R.
Title: Genome specific PPARαB duplicates in salmonids and insights into estrogenic regulation in brown trout
Publisher: Elsevier
Issue Date: 2017
Abstract: Peroxisome proliferator-activated receptors (PPARs) are key regulators of many processes in vertebrates, such as carbohydrate and lipid metabolism. PPARα, a member of the PPAR nuclear receptor gene subfamily (NR1C1), is involved in fatty acid metabolism, namely in peroxisomal β-oxidation. Two gene paralogues, pparαA and pparαB, were described in several teleost species with their origin dating back to the teleost-specific genome duplication (3R). Given the additional salmonid-specific genome duplication (4R), four genes could be theoretically anticipated for this gene subfamily. In this work, we examined the pparα gene repertoire in brown trout, Salmo trutta f. fario. Data disclosed two pparα-like sequences in brown trout. Phylogenetic analyses further revealed that the isolated genes are most likely genome pparαB duplicates, pparαBa and pparαBb, while pparαA is apparently absent in salmonids. Both genes showed a ubiquitous mRNA expression across a panel of 11 different organs. In vitro exposed primary brown trout hepatocytes strongly suggest that pparα gene paralogues are differently regulated by ethinylestradiol (EE2). PparαBb mRNA expression significantly decreased with dosage, reaching significance after exposure to 50 μM EE2, while pparαBa mRNA increased, significant at 1 μM EE2. The present data enhances the understanding of pparα function and evolution in teleost, and reinforces the evidence of a potential crosstalk between estrogenic and pparα signaling pathways. © 2017 Elsevier
Subject: ethinylestradiol
peroxisome proliferator activated receptor alpha
estrogen
peroxisome proliferator activated receptor
adult
amino acid sequence
animal cell
Article
carbohydrate metabolism
controlled study
fatty acid oxidation
female
in vitro study
liver cell
male
nonhuman
organ distribution
phylogeny
priority journal
real time polymerase chain reaction
Salmo trutta
synteny
animal
cell culture
cytology
drug effects
gene expression regulation
genetics
genome
growth, development and aging
metabolism
salmonine
Animals
Cells, Cultured
Estrogens
Gene Expression Regulation
Genome
Hepatocytes
Peroxisome Proliferator-Activated Receptors
Phylogeny
Real-Time Polymerase Chain Reaction
Trout
URI: https://hdl.handle.net/10216/120373
Source: Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology, vol. 208-209, p. 94-101
Related Information: info:eu-repo/grantAgreement/FCT/5876/147268/PT
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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