Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120360
Author(s): Lopes C.
Madureira T.V.
Ferreira N.
Pinheiro I.
Castro L.F.C.
Rocha E.
Title: Peroxisome proliferator-activated receptor gamma (PPARγ) in brown trout: Interference of estrogenic and androgenic inputs in primary hepatocytes
Publisher: Elsevier
Issue Date: 2016
Abstract: Peroxisome proliferator-activated receptor gamma (PPARγ) is a pivotal regulator of lipid and glucose metabolism in vertebrates. Here, we isolated and characterized for the first time the PPARγ gene from brown trout (Salmo trutta f. fario). Hormones have been reported to interfere with the regulatory function of PPARγ in various organisms, albeit with little focus on fish. Thus, primary hepatocytes isolated from juveniles of brown trout were exposed to 1, 10 and 50 μM of ethinylestradiol (EE2) or testosterone (T). A significant (3 fold) decrease was obtained in response to 50 μM of EE2 and to 10 and 50 μM of T (13 and 14 folds), while a 3 fold increase was observed at 1 μM of EE2. Therefore, trout PPARγ seems a target for natural/synthetic compounds with estrogenic or androgenic properties and so, we advocate considering PPARγ as another alert sensor gene when assessing the effects of sex-steroid endocrine disruptors. © 2016 Elsevier
Subject: androgen
estrogen
ethinylestradiol
peroxisome proliferator activated receptor gamma
testosterone
amino acid sequence
animal
cell culture
chemistry
dose response
drug effects
genetics
liver cell
metabolism
phylogeny
primary cell culture
salmonine
sequence alignment
Amino Acid Sequence
Androgens
Animals
Cells, Cultured
Dose-Response Relationship, Drug
Estrogens
Ethinyl Estradiol
Hepatocytes
Phylogeny
PPAR gamma
Primary Cell Culture
Sequence Alignment
Testosterone
Trout
URI: https://hdl.handle.net/10216/120360
Source: Environmental Toxicology and Pharmacology, vol. 46, p. 328-336
Related Information: info:eu-repo/grantAgreement/FCT/5876/147268/PT
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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