Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120348
Author(s): Guzmán-Guillén R.
Prieto Ortega A.I.
Moreno I.M.
Ríos V.
Moyano R.
Blanco A.
Vasconcelos V.
Cameán A.M.
Title: Effects of depuration on histopathological changes in tilapia (Oreochromis niloticus) after exposure to cylindrospermopsin
Publisher: John Wiley and Sons
Issue Date: 2017
Abstract: Cylindrospermopsin (CYN) is a highly water-soluble cytotoxin produced by several species of freshwater cyanobacteria and it is considered the second most studied cyanotoxin worldwide. CYN acts as a potent protein and glutathione synthesis inhibitor, as well as inducing genotoxicity, oxidative stress and histopathological alterations. Studies concerning the depuration of cyanobacterial toxins in aquatic organisms, especially in fish, are of great interest for fish economy and public health, but are scarce in the case of CYN. This is the first study reporting the ability of depuration (3 − 7 days) in reversing or ameliorating the histopathological lesions induced in liver, kidney, heart, intestines, and gills of tilapia (Oreochromis niloticus) due to exposure by immersion to repeated doses of a CYN-containing culture of A. ovalisporum for 14 days. The main histopathological changes induced by CYN were glucogenic degeneration and loss of the normal hepatic cord-structure (liver), hyperemia, dilated Bowman's capsule and cellular tumefaction (kidney), myofibrolysis, hemorrhages and edema (heart), necrosis and partial loss of microvilli (gastrointestinal tract), and hyperemia and inflammatory cells infiltrates (gills). After 3 days of depuration, gills were totally recovered, while the liver, kidney, and gastrointestinal tract required 7 days, and longer depuration periods may be needed for a full recovery of the heart. In addition, the morphometric study indicated that depuration managed to reverse the affectation in the hepatocytes nuclear diameters and cross sections of the proximal and distal convoluted tubules induced in CYN-exposed fish. In general, these results validate depuration as an effective practice for detoxification of fish contaminated with CYN. © 2016 Wiley Periodicals, Environ Toxicol 32: 1318–1332, 2017. © 2016 Wiley Periodicals,
Subject: Aquatic organisms
Biosynthesis
Detoxification
Cylindrospermopsin
Depuration
Histopathology
Morphometry
Tilapia
Fish
cylindrospermopsin
bacterial toxin
cylindrospermopsin
uracil
anatomy
cichlid
cyanobacterium
detoxification
genotoxicity
histopathology
inhibitor
lesion
morphometry
oxidative stress
protein
toxin
animal tissue
Aphanizomenon ovalisporum
Article
autecology
bleeding
Bowman gland
cell infiltration
contaminated fish
controlled study
convalescence
cyanobacterium
depuration
gill
glucogenesis
heart edema
heart injury
heart tissue
histopathology
hyperemia
immersion
inflammatory cell
intestine injury
intestine necrosis
intestine tissue
kidney distal tubule
kidney injury
kidney proximal tubule
kidney tissue
liver cell
liver injury
liver tissue
male
microvillus
nonhuman
Oreochromis niloticus
priority journal
sanitation
tissue characterization
tissue injury
analogs and derivatives
animal
cytology
drug effects
intestine
kidney
liver
metabolism
oxidative stress
pathology
scanning electron microscopy
Tilapia
transmission electron microscopy
Cyanobacteria
Oreochromis niloticus
Tilapia
Animals
Bacterial Toxins
Cyanobacteria
Gills
Hepatocytes
Intestines
Kidney
Liver
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Oxidative Stress
Tilapia
Uracil
URI: https://hdl.handle.net/10216/120348
Source: Environmental Toxicology, vol. 32(4), p. 1318-1332
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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