Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120338
Author(s): Frazão B.
Campos A.
Osório H.
Thomas B.
Leandro S.
Teixeira A.
Vasconcelos V.
Antunes A.
Title: Analysis of Pelagia noctiluca proteome Reveals a Red Fluorescent Protein, a Zinc Metalloproteinase and a Peroxiredoxin
Publisher: Springer Verlag (Germany)
Issue Date: 2017
Abstract: Pelagia noctiluca is the most venomous jellyfish in the Mediterranean Sea where it forms dense blooms. Although there is several published research on this species, until now none of the works has been focused on a complete protein profile of the all body constituents of this organism. Here, we have performed a detailed proteomics characterization of the major protein components expressed by P. noctiluca. With that aim, we have considered the study of jellyfish proteins involved in defense, body constituents and metabolism, and furthered explore the significance and potential application of such bioactive molecules. P. noctiluca body proteins were separated by1D SDS–PAGE and 2DE followed by characterization by nanoLC-MS/MS and MALDI-TOF/TOF techniques. Altogether, both methods revealed 68 different proteins, including a Zinc Metalloproteinase, a Red Fluorescent Protein (RFP) and a Peroxiredoxin. These three proteins were identified for the first time in P. noctiluca. Zinc Metalloproteinase was previously reported in the venom of other jellyfish species. Besides the proteins described above, the other 65 proteins found in P. noctiluca body content were identified and associated with its clinical significance. Among all the proteins identified in this work we highlight: Zinc metalloproteinase, which has a ShK toxin domain and therefore should be implicated in the sting toxicity of P. noctiluca.; the RFP which are a very important family of proteins due to its possible application as molecular markers; and last but not least the discovery of a Peroxiredoxin in this organism makes it a new natural resource of antioxidant and anti-UV radiation agents. © 2017, Springer Science+Business Media New York.
Subject: heat shock protein
peroxiredoxin
proteome
red fluorescent protein
coelenterate venom
metalloproteinase
peroxiredoxin
photoprotein
proteome
red fluorescent protein
ShK neurotoxin
zinc
Article
controlled study
DNA sequence
liquid chromatography-mass spectrometry
matrix assisted laser desorption ionization time of flight mass spectrometry
nonhuman
Pelagia noctiluca
poisonous jellyfish
polyacrylamide gel electrophoresis
protein expression
proteomics
separation technique
two dimensional electrophoresis
animal
chemistry
electrophoresis
electrospray mass spectrometry
jellyfish
Mediterranean Sea
metabolism
protein domain
tandem mass spectrometry
Animals
Cnidarian Venoms
Electrophoresis
Luminescent Proteins
Mediterranean Sea
Metalloproteases
Peroxiredoxins
Protein Domains
Proteome
Scyphozoa
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry
Zinc
URI: https://hdl.handle.net/10216/120338
Source: Protein Journal, vol. 36(2), p. 77-97
Related Information: info:eu-repo/grantAgreement/FCT/5876/147268/PT
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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