Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120328
Author(s): Fernandes J.P.
Almeida C.M.R.
Andreotti F.
Barros L.
Almeida T.
Mucha A.P.
Title: Response of microbial communities colonizing salt marsh plants rhizosphere to copper oxide nanoparticles contamination and its implications for phytoremediation processes
Publisher: Elsevier
Issue Date: 2017
Abstract: This study aimed to investigate Cu oxide nanoparticles (CuO NP) effect on microbial communities associated with salt marsh plants (Halimione portulacoides and Pragmites australis) rhizosphere and its implications for phytoremediation processes. Experiments were conducted, under controlled conditions, over one week. Rhizosediment soaked in the respective elutriate (a simplified natural medium) with or without plants, was doped with CuO NP or with Cu in ionic form. Microbial community in rhizosediments was characterized in terms of abundance (by DAPI) and structure (by ARISA). Metal uptake by plants was evaluated by measuring Cu in plant tissues (by atomic absorption spectroscopy). Results revealed significant metal uptake but only in plant roots, which was significantly lower (H. portulacoides) or not significant (P. australis) when the metal was in NP form. Microbial community structure was significantly changed by the treatment (absence/presence of Cu, ionic Cu or CuO NP) as showed by multivariate analysis of ARISA profiles and confirmed by analysis of similarities (Global test - one way ANOSIM). Moreover, in P. australis rhizosediments microbial abundance, bacterial richness and diversity indexes were significantly affected (increased or decreased) due to metal presence whereas in H. portulacoides rhizosediment microbial abundance showed a significant decrease, particularly when the metal was in NP form. Accordingly, Cu presence affected the response of the rhizosphere microbial community and in some cases that response was significantly different when Cu was in NP form. The response of the microbial communities to Cu NP might also contribute to the lower metal accumulation by plants when the metal was in this form. So, Cu NP may cause disturbances in ecosystems functions, ultimately affecting phytoremediation processes. These facts should be considered regarding the use of appropriate salt marshes plants to remediate moderately impacted areas such as estuaries, where NPs can be found. © 2017 Elsevier
Subject: Absorption spectroscopy
Bioremediation
Copper
Copper oxides
Ecology
Ecosystems
Microorganisms
Multivariant analysis
Nanoparticles
Remediation
Social sciences
Soils
Wetlands
Atomic absorption spectroscopy
Bacterial community structure
Copper oxide nanoparticles
Halimione portulacoides
Microbial community structures
Multi variate analysis
Phytoremediation
Rhizosphere microbial communities
Metals
copper
copper ion
copper oxide nanoparticle
community structure
copper compound
microbial community
nanoparticle
phytoremediation
plant
pollutant
rhizosphere
saltmarsh
Article
atomic absorption spectrometry
bioaccumulation
community structure
controlled study
estuary
Halimione portulacoides
microbial colonization
microbial community
microbial diversity
nonhuman
phytoremediation
plant metabolism
plant tissue
population abundance
Pragmites australis
rhizosphere
rhizospheric microorganism
salt marsh
species richness
vascular plant
Bacteria (microorganisms)
Halimione portulacoides
Phragmites australis
URI: https://hdl.handle.net/10216/120328
Source: Science of the Total Environment, vol. 581-582, p. 801-810
Related Information: info:eu-repo/grantAgreement/FCT/5876/147268/PT
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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