Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120326
Author(s): Fernandes F.
Barbosa M.
Pereira D.M.
Sousa-Pinto I.
Valentão P.
Azevedo I.C.
Andrade P.B.
Title: Chemical profiling of edible seaweed (Ochrophyta) extracts and assessment of their in vitro effects on cell-free enzyme systems and on the viability of glutamate-injured SH-SY5Y cells
Publisher: Elsevier
Issue Date: 2018
Abstract: Neurodegenerative processes involve numerous and closely related events that ultimately culminate in neuronal cell injury. The aim of this study was (i) to assess, for the first time, the neuroprotective potential of acetone extracts of six edible species of Ochrophyta, by evaluating their cholinesterase and lipoxygenase inhibitory activity in cell-free assays, as well as their capacity to attenuate glutamate-induced toxicity in neuronal (SH-SY5Y) cells, and (ii) to try to relate the chemical composition of the extracts with their biological activity, evaluating also the effect of the main compounds thereof. In spite of a modest cholinesterase inhibition, a dose-dependent response towards lipoxygenase was found for all macroalgae extracts. At non-cytotoxic concentrations, the extracts from Fucus serratus Linnaeus and Saccharina latissima (Linnaeus) C.E. Lane, C. Mayes, Druehl & G.W. Saunders were able to improve the viability of glutamate-insulted SH-SY5Y cells. These results encourage further studies for a more detailed understanding of the mechanisms beyond the documented biological activities, and point to the potential interest of the selected seaweed species and their extracts as promising candidates for in vivo studies. © 2018 Elsevier
Subject: acetone
algal extract
arachidonate 5 lipoxygenase
carotenoid
chlorophyll
cholinesterase
fatty acid
lipoxygenase
neuroprotective agent
Ochrophyta extract
unclassified drug
arachidonate 5 lipoxygenase
cholinesterase
enzyme inhibitor
fucoxanthin
glutamic acid
neuroprotective agent
oleic acid
palmitic acid
plant extract
xanthophyll
Article
cell viability
chemical composition
concentration response
controlled study
cytotoxicity
drug activity
drug determination
drug effect
drug mechanism
enzyme inhibition
Fucus serratus
high performance liquid chromatography
human
human cell
in vitro study
neuroprotection
neurotoxicity
SH-SY5Y cell line
cell free system
cell survival
chemistry
dose response
edible plant
enzymology
mass fragmentography
nerve cell
seaweed
tumor cell line
Arachidonate 5-Lipoxygenase
Cell Line, Tumor
Cell Survival
Cell-Free System
Cholinesterases
Chromatography, High Pressure Liquid
Dose-Response Relationship, Drug
Enzyme Inhibitors
Gas Chromatography-Mass Spectrometry
Glutamic Acid
Humans
Neurons
Neuroprotective Agents
Oleic Acid
Palmitic Acid
Plant Extracts
Plants, Edible
Seaweed
Xanthophylls
URI: https://hdl.handle.net/10216/120326
Source: Food and Chemical Toxicology, vol. 116, p. 196-206
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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