Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/120284
Author(s): | Bondoso J. Godoy-Vitorino F. Balagué V. Gasol J.M. Harder J. Lage O.M. |
Title: | Epiphytic Planctomycetes communities associated with three main groups of macroalgae |
Publisher: | Oxford University Press |
Issue Date: | 2017 |
Abstract: | Planctomycetes, a unique group of widespread and understudied bacteria, are known to be associated with macroalgae. The temporal dynamics and the host-specific association of planctomycetal communities on Fucus spiralis, Ulva sp. and Chondrus crispus from two locations in the North Coast of Portugal were assessed both by denaturing gradient gel electrophoresis with group-specific primers and 16S rDNA amplicon libraries. The epiphytic planctomycetal communities showed a significant association with the host macroalgal species independently of the geographical location and the season. This pattern was confirmed by clone libraries of winter and summer samples: we obtained 720 16S rRNA gene sequences that represented 44 operational taxonomic units (OTUs) within the phylum Planctomycetes. Most of the OTUs belonged to Blastopirellula, followed by Rhodopirellula, Planctomyces, the Pir4 lineage and the uncultured class OM190 (this last one nearly 30% of the OTUs). Ulva sp. and C. crispus had more diverse planctomycetal communities than F. spiralis. Analysis of beta diversity showed that the planctomycetal microbiome was host specific. We hypothesize that the specific association of Planctomycetes and their macroalgal hosts is likely determined by nutritional molecules provided by the algae and the set of sulfatases inherent to each Planctomycetes species. © FEMS 2017. |
Subject: | bacterium electrokinesis enzyme enzyme activity epiphyte gene gene expression genetic analysis macroalga RNA seasonal variation Portugal algae Blastopirellula Chondrus crispus Fucus spiralis Otus Planctomyces Planctomycetes Rhodopirellula Ulva primer DNA ribosome DNA RNA 16S bacterium denaturing gradient gel electrophoresis genetics host range microbiology phylogeny physiology Planctomycetales Portugal seaweed Bacteria Denaturing Gradient Gel Electrophoresis DNA Primers DNA, Ribosomal Host Specificity Phylogeny Planctomycetales Portugal RNA, Ribosomal, 16S Seaweed |
URI: | https://hdl.handle.net/10216/120284 |
Source: | FEMS Microbiology Ecology, vol. 93(3): fiw255 |
Related Information: | info:eu-repo/grantAgreement/FCT/5876/147268/PT |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | restrictedAccess |
Appears in Collections: | CIIMAR - Artigo em Revista Científica Internacional |
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File | Description | Size | Format | |
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Bondoso J_2017.pdf Restricted Access | 1.11 MB | Adobe PDF | Request a copy |
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