Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120273
Author(s): Antas P.
Carneiro M.
Reis B.
Castelo-Branco R.
Azevedo J.
Urbatzka R.
Campos A.
Vasconcelos V.
Martins J.C.
Title: GST transcriptional changes induced by a toxic Microcystis aeruginosa strain in two bivalve species during exposure and recovery phases
Publisher: Springer International Publishing
Issue Date: 2018
Abstract: Previous studies have demonstrated the modulation of glutathione transferases (GSTs) induced by microcystin (MC) alone or in combination with other cyanobacterial secondary metabolites in bivalves. However, interspecies information about which and how GST isoforms are affected by these secondary metabolites is still scarce, especially considering the dynamic process involving their uptake and elimination routes. In this context, the role of GSTs gene expression changes in response to a toxic Microcystis aeruginosa extract were examined for Mytilus galloprovincialis and Ruditapes philippinarum during exposure and recovery phases. The expression levels of sigma 1, sigma 2, pi and mu-class GST genes were analyzed in the hepatopancreas of both bivalve species during cyanobacteria extract exposure (24 h) and post-exposure (24 and 72 h). Only a significant induction of sigma 1-class GST expression was observed for R. philippinarum upon 24-hour exposure of both bivalve species to Microcystis extract. During the recovery phase, GST transcriptional changes for M. galloprovincialis were characterized by an early induction (24 h) of sigma 1 and sigma 2 transcripts. On the other hand, GST transcriptional changes for R. philippinarum during post-exposure phase were characterized by an early induction (24 h) of sigma 1 and mu transcripts and a later induction (72 h) of the four analyzed GST transcripts. Such differences reflect variable GST response mechanisms to cope with MC-producing cyanobacterial blooms exposure between these two bivalve species, revealing a higher sensitivity of R. philippinarum to Microcystis-induced stress than M. galloprovincialis. The results also suggest a much higher level of activity of the GST detoxification system during the recovery phase compared to the period of the stress exposure for both bivalve species. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.
Subject: bacterial toxin
glutathione transferase
glutathione transferase mu
glutathione transferase sigma 1
glutathione transferase sigma 2
unclassified drug
glutathione transferase
microcystin
algal bloom
bivalve
cyanobacterium
gene expression
metabolite
oxidative stress
pollution exposure
toxicology
Article
bacterial overgrowth
bacterial strain
bivalve
chemical stress
controlled study
cyanobacterium
detoxification
DNA modification
gene expression
gene induction
genetic analysis
genetic transcription
GST mu gene
GST sigma 1 gene
GST sigma 2 gene
hepatopancreas
Microcystis aeruginosa
Mytilus galloprovincialis
nonhuman
priority journal
protein expression
Ruditapes philippinarum
species difference
animal
drug effect
genetics
Microcystis
Mytilus
physiology
toxicity
toxicity testing
water pollutant
Bivalvia
Cyanobacteria
Microcystis
Microcystis aeruginosa
Mytilus galloprovincialis
Venerupis (Ruditapes) philippinarum
Animals
Glutathione Transferase
Hepatopancreas
Microcystins
Microcystis
Mytilus
Toxicity Tests
Water Pollutants, Chemical
URI: https://hdl.handle.net/10216/120273
Source: Ecotoxicology, vol. 27(9), p. 1272-1280
Related Information: info:eu-repo/grantAgreement/FCT/5876/147268/PT
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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